GENETIC DIVERSITY OF GUAVA GENOTYPES EVALUATED USING RAPD MOLECULAR MARKER

B. SHIVA¹*, A. NAGARAJA², RAKESH SINGH³, MANISH SRIVASTAV^4
1ICAR-Indian Agricultural Research Institute, Pusa, New Delhi, Delhi 110012, India
²ICAR-Indian Agricultural Research Institute, Pusa, New Delhi, Delhi 110012, India
³ICAR- Nation Bureau of Plant Genetic Resources, New Delhi, 110 012, India
⁴ICAR-Indian Agricultural Research Institute, Pusa, New Delhi, Delhi 110012, India
* Corresponding Author : banothshivaiari@gmail.com

Received : 08-05-2017     Accepted : 15-05-2017     Published : 28-05-2017
Volume : 9     Issue : 5       Pages : 271 - 274
Genetics 9.5 (2017):271-274

Keywords : Characterization, Polymorphic Information Content, Jacards similarity matrix, Genetic Similarity, RAPD
Academic Editor : Prashant Kisanrao Nimbolkar
Conflict of Interest : None declared
Acknowledgements/Funding : I express my sincere thanks to the Head, Division of Fruits and Horticultural Technology, ICAR- IARI, New Delhi, for providing me support and technical guidance in all course of my field and lab work
Author Contribution : All author equally contributed

Genetic diversity among 24 genotypes (22 varieties/collection belonging to Psidium guajava and 2 other species) of guava were characterized using Random Amplified Polymorphic DNA (RAPD) markers. Out of 29 RAPD primers used, 10 were found to be monomorphic and 19 showed polymorphism among guava genotypes. Number of alleles detected using polymorphic RAPD primer ranged between 2 (OPA13A) to 11 (OPF02A) with an average of 6 amplicons/primer. High rate of polymorphism was observed reasonably for OPF02A, OPH19A, OPF13A, OPA13A and OPB13A primers. The PIC value ranged from 0.49-0.89 indicates that the markers were quite informative. Based on molecular analysis, Sasri Selection and Sasni Selection were grouped with Allahabad Safeda. Tamil Nadu Selection and Lalit genotypes formed a group at 50% similarity. Psidum freidrichsthalianum formed a group with black guava. Molecular analysis showed a high degree of variation among analyzed guava genotypes indicating an important source of genetic diversity that can be used in the guava improvement program

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