ESTIMATION OF POPULATION AND EVALUATION OF RHIZOSPHERE MYCOFLORA AND FLUORESCENT PSEUDOMONADS AGAINST Rhizoctonia solani UNDER IN VITRO CONDITION

N. SUMALATHA¹*, B. PUSHPAVATHI², R. JAGADEESHWAR³, V. SURESH⁴, R.V.S.K. REDDY^5
1Department of Plant Pathology, Prof Jayashankar Telangana State Agricultural University, Rajendranagar, Hyderabad, 500030, India
²Department of Plant Pathology, Prof Jayashankar Telangana State Agricultural University, Rajendranagar, Hyderabad, 500030, India
³Department of Plant Pathology, Prof Jayashankar Telangana State Agricultural University, Rajendranagar, Hyderabad, 500030, India
⁴Department of Plant Pathology, Prof Jayashankar Telangana State Agricultural University, Rajendranagar, Hyderabad, 500030, India
⁵Department of Plant Pathology, Prof Jayashankar Telangana State Agricultural University, Rajendranagar, Hyderabad, 500030, India
* Corresponding Author : suma.nallabeema@gmail.com

Received : 01-02-2017     Accepted : 22-02-2017     Published : 28-02-2017
Volume : 9     Issue : 2       Pages : 857 - 860
Int J Microbiol Res 9.2 (2017):857-860

Keywords : Rhizosphere isolates, Rhizoctonia solani, Root rot, Pseudomonads and Trichoderma
Academic Editor : Deepak Kumar, Dr Vijay Kumar
Conflict of Interest : None declared
Acknowledgements/Funding : , N. Sumalatha, gratefully acknowledge the support of Department Plant Pathology, College of Agriculture, Rajendranagar, Hyderabad, Prof. Jayashankar Telangana State Agricultural University (PJTSAU) for providing all the facilities during the research work
Author Contribution : None declared

Tomato crop is attacked by several soil borne fungal pathogens among which Rhizoctonia solani is the most important soil borne pathogen causing symptoms of damping off, crown rot and root rot. To provide bio-efficacy against casual agent, the population of rhizosphere mycoflora and fluorescent pseudomonads was estimated from the soil samples collected from diseased, healthy and luxuriously growing weed plants as colony forming units (cfu) on different culture media and were tested for their antagonistic potential against R. solani under in vitro condition. Among the sources of rhizosphere soil collected, significantly highest mean cfu count of mycoflora (27.70) and fluorescent pseudomonads (40.75) was observed in case of samples collected from weed plants followed by healthy tomato plants. Among the mycoflora, the isolate M10 was found to be the potential antagonist and was identified as Trichoderma viride, whereas the effective pseudomonad isolate P1 was identified as Pseudomonas fluorescens.