ISOLATION, CHARACTERIZATION, SEROTYPING AND ANTIBIOGRAM STUDIES OF E.coli COLLECTED FROM DIARRHOEIC NEONATAL KIDS

HARDIK PATEL¹, I.H. KALYANI², AKSHAY BARIYA³*, VAIBHAV DODIYA⁴, PRAMOD SAKHARE⁵, KISHAN SHARMA^6
1Department of Veterinary Microbiology, Vanbandhu College of Veterinary Science and Animal Husbandry, Navsari Agricultural University, Navsari, Gujarat, India
²Department of Veterinary Microbiology, Vanbandhu College of Veterinary Science and Animal Husbandry, Navsari Agricultural University, Navsari, Gujarat, India
³College of Veterinary Science and Animal Husbandry, Junagadh Agricultural University, Junagadh, Gujarat, India
⁴College of Veterinary Science and Animal Husbandry, Junagadh Agricultural University, Junagadh, Gujarat, India
⁵Department of Veterinary Microbiology, Vanbandhu College of Veterinary Science and Animal Husbandry, Navsari Agricultural University, Navsari, Gujarat, India
⁶Department of Veterinary Microbiology, Vanbandhu College of Veterinary Science and Animal Husbandry, Navsari Agricultural University, Navsari, Gujarat, India
* Corresponding Author : akshaybariya196@gmail.com

Received : 24-01-2017     Accepted : 04-02-2017     Published : 28-02-2017
Volume : 9     Issue : 2       Pages : 854 - 856
Int J Microbiol Res 9.2 (2017):854-856

Keywords : Kids diarrhoea, E.coli, Isolation, Characterization, Antibiogram
Academic Editor : Dr Milan M. Chudasama
Conflict of Interest : None declared
Acknowledgements/Funding : We are grateful to the Director of Research and Dean P.G studies and Dean and Principal College of Veterinary Science and Animal Husbandry for providing research facilities and funding
Author Contribution : All authors are equally contributing in research design and planed a study

Neonatal diarrhoea is a major threat responsible for high mortality in neonates particularly during the first week after birth. The present study was undertaken to find out the prevalence of Escherichia coli (E. coli) with neonatal kid diarrhoea. A total of 103 faecal samples were collected from diarrhoeic goat kids up to 4 weeks of age and processed for detection of E. coli by cultural and biochemical techniques. The enteropathogens detected E. coli 75 (72.81%) were recovered producing lactose fermenting pink colored colonies on MacConkey agar medium and colonies with greenish metallic sheen on EMB agar medium. Further, all the 75 isolates showed typical IMViC patterns of E. coli viz. Indole and Methyl- red positive, Voges Proskauer and Citrate negative. Out of 75 isolates, 35 isolates were sent to National Salmonella and Escherichia Center (NSEC), Central Research Institute, Kasauli. Of these 35 isolates, 22 were serotyped into nine different ‘O’ groups, while two isolates were untypable and six isolates were found rough. The serotypes obtained were O2, O84, O86, O87, O101, O118, O120, O128, O141 and O157. The most predominant serotypes were O120 (nine isolates), O141 (four isolates) and O157 (two isolates) while other serotypes were O2, O84, O86, O87, O101, O118 and O128 (one isolate each). Out of 75 isolates, very high resistance was showed against Cephalexin 72 isolates (96.00%), Kanamycin 70 isolates (93.33%). Moderate number of isolates were found to be resistant to Tetracycline 45 isolates (60.00%), while low prevalence of resistance showed against Ciprofloxacin 25 isolates (33.33%), Trimethoprim 16 isolates (21.33%), 15 isolates (20.00%) to Gatifloxacin and 12 isolate (16.00%) to Enrofloxacin.