SYNTHESIS, CHARACTERIZATION AND SCREENING OF THIAZOLIDINE-2,4-DIONE DERIVATIVES AS ANTIMICROBIAL AGENTS

KARUNA S. SHUKLA¹*, POOJA CHAWLA², SHAILENDRA PANDEY^3
1Pharmaceutical Research Laboratory, School of Pharmacy, Babu Banarasi Das University, Lucknow
²Gyani Inder Singh Institute of Professional Studies, Dehradun, Uttarakhand
³Department of Pharmacy, Sarojani Naidu Medical College, Agra
* Corresponding Author : spandey.rs.phe@iitbhu.ac.in

Received : 27-09-2016     Accepted : 29-10-2016     Published : 28-11-2016
Volume : 8     Issue : 12       Pages : 807 - 812
Int J Microbiol Res 8.12 (2016):807-812

Keywords : Antimicrobial agents, Thiazolidinedione, Molecular sieves and minimum inhibitory concentration
Academic Editor : Akash Ved
Conflict of Interest : None declared
Acknowledgements/Funding : One of the authors is greatly acknowledge Institute of Microbial Technology, Chandigarh, India for providing microbial strains. Authors would like to acknowledge Dean School of Pharmacy BabuBanarasi Das University, Lucknow for providing necessary facilities during research
Author Contribution : None declared

Thiazolidine-2,4-dione derivatives (4a-4e) have been synthesized and characterized by physico-chemical, elemental (C,H,N,S), FT-IR, mass and 1HNMR spectral analysis. In this context, the aim was to synthesized thiazolidine-2,4-dione derivatives and were screened for antimicrobial activity. Antimicrobial activity was screened using agar well diffusion assay method for determination of the diameter of zone of inhibition in millimeter (mm) and solid dilution method for determination of minimum inhibitory concentration (MIC) in µg/ml against Gram-positive(Staphylococcus aureus MTCC 1430, Bacillus subtilis MTCC 0441), Gram- negative(Escherichia coli MTCC 1573, Pseudomonas aeruginosa MTCC 2453) and fungal strains (Aspergillus tubingensis MTCC 2546). The antimicrobial activity of compound 4c2-(4-((2,4-dioxothiazolidin-5-ylidene)methyl)phenoxy)-N-(4-fluorophenyl)acetamidewas found to be most potent against all the tested strains of microorganisms with the zone of inhibition 17.1-19.5 mm at 200µg/ml and minimum inhibitory concentration value 0.6-0.8 µg/ml.