MOLECULAR CHARACTERIZATION OF GROWTH HORMONE RELEASING HORMONE RECEPTOR (GHRHR): CLONING, TISSUE DISTRIBUTION, EXPRESSION ANALYSIS AND HOMOLOGY MODELING IN FARMED CARP, Labeo rohita

M. MOHANTY¹, S. PATNAIK², S.K. PATRA³, L. SAHOO⁴, P. DAS⁵, H.K. BARMAN⁶, P. JAYASANKAR⁷*
¹Fish Genetics and Biotechnology Division, ICAR-Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, 751002, Odisha, India
²Fish Genetics and Biotechnology Division, ICAR-Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, 751002, Odisha, India
³Fish Genetics and Biotechnology Division, ICAR-Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, 751002, Odisha, India
⁴Fish Genetics and Biotechnology Division, ICAR-Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, 751002, Odisha, India
⁵Fish Genetics and Biotechnology Division, ICAR-Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, 751002, Odisha, India
⁶Fish Genetics and Biotechnology Division, ICAR-Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, 751002, Odisha, India
⁷Fish Genetics and Biotechnology Division, ICAR-Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, 751002, Odisha, India
* Corresponding Author : P.Jayasankar@icar.gov.in

Received : 07-10-2016     Accepted : 23-10-2016     Published : 28-10-2016
Volume : 8     Issue : 6       Pages : 215 - 221
Genetics 8.6 (2016):215-221

Keywords : GHRHR, growth stages, Labeo rohita, mRNA expression, qPCR
Academic Editor : Dr K M Singh, Panda Rudra Prasanna
Conflict of Interest : None declared
Acknowledgements/Funding : Financial assistance by Department of Science and Technology (DST) and Indian Council of Agricultural Research (ICAR) is highly acknowledged. The authors are also grateful to The Director, ICAR-CIFA for providing laboratory facilities. The authors are thankful to Dr. K. D. Mahapatra, Principal scientist, ICAR-CIFA for providing the experimental samples. Technical supports of all the members of Fish Genetics and Biotechnology Division is also avowed.
Author Contribution : None declared

Growth hormone releasing hormone receptor (GHRHR) is a trans-membrane receptor belonging to the Class-II GPCR family. GHRHR activates growth-hormone production consequent upon triggering somatotroph proliferation. Unlike in mammals, information on teleosts GHRHR is insufficient. Here, GHRHR cDNA has been cloned from the brain tissue of Labeo rohita, a large-bodied farmed carp. It is a 1320 bp transcript consisting of an ORF (open reading frame) translatable to a 416aa long polypeptide. Relative mRNA expressions of GHRHR in various organs of different age groups (i.e. juvenile, young and adult) were estimated by using q-RT PCR analyses. Phylogenetically GHRHR was closer to Cyprinidae family. It was found to be 92-93% similar to Carassius auratus followed by Danio rerio. It was abundantly expressed in the pituitary and brain followed by male/female gonads of all age groups in rohu; demonstrating its possible functions may not be restricted to GH release only, but also in many other developmental and physiological regulations. The predicted stable 3D structure and protein-protein interactions provided the clue about its diversified networking roles. Our findings could be of great help for future studies to delineate its multidirectional neuroendocrine roles in various organs in teleosts.

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