ISSN : 0975-5276
EISSN : 0975-9174
G. KRANTHI KUMAR1, M. RAGHU RAM2*
1Department of Botany & Microbiology, Acharya Nagarjuna University, Guntur, 522510, Andhra Pradesh, India
2Department of Botany & Microbiology, Acharya Nagarjuna University, Guntur, 522510, Andhra Pradesh, India
* Corresponding Author : mraghuram2002@gmail.com
Received : 03-07-2016 Accepted : 09-08-2016 Published : 14-08-2016
Volume : 8 Issue : 8 Pages : 781 - 784
Int J Microbiol Res 8.8 (2016):781-784
Keywords : PGPR, Indole Acid Acetic, Chitinase, Exo polysaccharide, HCN, Siderophore
Conflict of Interest : None declared
Acknowledgements/Funding : The authors are thankful to UGC, New Delhi for the financial assistance under Major Research Project (No. F. 37-5/2009). Kranthi Kumar is thankful to UGC for the fellowship as project fellow.
Author Contribution : None declared
In the present study, six rhizobial strains were isolated from root nodules of Vigna trilobata plants raised in soils collected from geographically different areas in A.P., India. All the isolates were biochemically characterized and screened in vitro for their plant growth promoting activities (PGP) viz. Indole Acetic Acid production, Chitinase production, Exopolysaccharide production, Siderophore and HCN production. These strains were identified as Agrobacterium tumefaciens MRR 108 (KC 415690), A. tumefaciens MRR 111 (KC 415692), Ensifer sp. MRR 101 (KC 428651), Ensifer fredii MRR 110 (KC 415691), Rhizobium sp. strain MRR 112(KF 621018) and Rhizobium sp. strain MRR 123 (KC 503884), after 16S rDNA sequencing. The results showed that the rhizobial isolates differ in the levels of PGP activities. All the six isolates were Indole Acetic Acid and EPS producers while Ensifer sp. MRR101 and Rhizobium sp. MRR 123 are siderophore producers and A. tumefaciens MRR 111 and Ensifer fredii MRR 110 are chitinase producers. Except, Ensifer fredii MRR 110 and Rhizobium sp. strain MRR 112 all are HCN producers. Therefore, these six strains with PGP potential are best biocontrol agents.