CELLULASE PRODUCING THERMOPHILIC BACTERIA FROM HOT SPRING OF ASSAM

PARVEEN ASSMA¹*, ARCHANA DEKA², GUNAJIT GOSWAMI³, MADHUMITA BAROOAH⁴, ROBIN CHANDRA BORO^5
1Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat, 785013, Assam
²Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat, 785013, Assam
³Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat, 785013, Assam
⁴Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat, 785013, Assam
⁵Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat, 785013, Assam
* Corresponding Author : assma@aau.ac.in

Received : 27-06-2016     Accepted : 04-08-2016     Published : 14-08-2016
Volume : 8     Issue : 8       Pages : 776 - 780
Int J Microbiol Res 8.8 (2016):776-780

Keywords : Thermophilic, cellulase, bacteria, cellulose, enzyme, endoglucanase
Conflict of Interest : None declared
Acknowledgements/Funding : None declared
Author Contribution : None declared

Thirty strains of bacteria were isolated from Garampani, under Nambor reserve forest in Golaghat district of Assam. Qualitative analysis was carried for the thirty isolates, though only eight isolates were found to have cellulose-degrading capacity. Analysis of the 16S rDNA sequences revealed degree of sequence similarity with Bacillus cereus, Bacillus thuringiensis, Stenotrophomonas maltophilia, Bacillus pumilus and Bacillus sp [3]. Growth curve analysis were done at 35°C, 45°C and 55°C. Thermal stability was evaluated at different temperatures ranging from 40°C-100°C as well as the stability of the enzymes were also assessed at different pH range of 2-10. Stenotrophomonas maltophilia (0.43 IU/ml) showed the highest cellulose activity followed by Bacillus cereus (0.39 IU/ml) and Bacillus thuringiensis (0.3 IU/ml) whereas Bacillus cereus (13.77 µg/ml) produced the highest amount of enzyme followed by and Bacillus sp (13.44µg/ml). Highlights • Eight isolates were found to have cellulose-degrading capacity • Bacterial isolates showed high degree of sequence similarity with Bacillus cereus, Bacillus thuringiensis, Stenotrophomonas maltophilia, Bacillus pumilus and Bacillus sp. • Stenotrophomonas maltophilia (0.43 IU/ml) showed the highest cellulase activity • Bacillus cereus (13.77 µg/ml) produced highest amount of enzyme