ISSN : 0975-5276
EISSN : 0975-9174
VIJAYA LAKSHMI GANGAVARAPU1*, JAYA MADHURI RAVURI2
1Department of Applied Microbiology, Sri Padmavati Mahila Visvavidyalayam, Tirupati, 517502, India
2Department of Applied Microbiology, Sri Padmavati Mahila Visvavidyalayam, Tirupati, 517502, India
* Corresponding Author : vijaya.gangavarapu@gmail.com , drjayaravuri@gmail.
Received : 17-03-2016 Accepted : 21-04-2016 Published : 07-05-2016
Volume : 8 Issue : 5 Pages : 754 - 758
Int J Microbiol Res 8.5 (2016):754-758
Keywords : Azo dyes, Recalcitrant Biodegradation, Decolourization, Molecular characterization
Academic Editor : K. V. Mohanan, Zeinab Shawky
Conflict of Interest : None declared
Acknowledgements/Funding : None declared
Author Contribution : Equal contribution was extended by both the authors in preparing, editing and submitting the manuscript.
Azo dyes are the synthetic dyes, which resist degradation and cause many environmental problems. Among different decolourizing microorganisms, bacteria are effective in degradation and complete mineralization of many dyes under optimal environmental conditions. In this research work, it was planned to examine the potential of a total of 22 newly isolated bacterial species for decolourization of azo dyes such as Reactive Violet 13 and Reactive Blue 171. Of these isolates, based on two levels of screening for their ability to degrade the selected dyes efficiently and rapidly, one isolate has been selected for further studies. The isolate showed rapid growth and decolourization of both dyes in LB than MS medium under specific growth conditions. Based on morphological, biochemical and molecular characterization (16s rRNA sequencing), the selected strain was identified as Pseudomonas stutzeri. It exhibited 70-80% decolourization of dyes at 100ppm concentration in Luria Bertani (LB) broth and 50-60% in Mineral Salt medium (MSM) within 24 h. An improved decolourization activity was observed with the increase in biomass concentration.