ISOLATION OF P- SOLUBILIZING BACTERIA FROM ACID SOILS OF ODISHA WITH ABILITY TO SOLUBILIZE INORGANIC PHOSPHATES

MADHUSMITA PRADHAN¹*, RANJAN KUMAR SAHOO², SANTANU MOHANTY³, CHINMAY PRADHAN^4
1Department of Soil Science & Agricultural Chemistry, College of Agriculture, OUAT, Bhubaneswar, Odisha, India
²Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, India
³Department of Soil Science & Agricultural Chemistry, College of Agriculture, OUAT, Bhubaneswar, Odisha, India
⁴Department of Botany, Utkal University, Bhubaneswar, Odisha, India
* Corresponding Author : madhusmita.pradhan47@gmail.com

Received : 11-04-2016     Accepted : 23-04-2016     Published : 28-04-2016
Volume : 8     Issue : 4       Pages : 747 - 753
Int J Microbiol Res 8.4 (2016):747-753

Keywords : Acid soil, PSB, AlPO4, FePO4.
Academic Editor : Ravi Ranjan
Conflict of Interest : None declared
Acknowledgements/Funding : We are thankful to SAIL, RSP, Rourkela, Odisha, India for partial financial support for carrying out the current piece of work.
Author Contribution : None declared

Soils vary in reaction, which directly correlates with availability of one of the major plant nutrients i.e. Phosphorous. Acid soil infertility mainly associated with fixed phosphates of Al and Fe, restrict the soluble P for crop uptake. Besides liming, P- solubilizing bacteria with the capacity to dissociate these Al and Fe bound P is the next viable alternative for sustainable agriculture. GPS based twenty (20) nos. of soil samples (pH ≤ 5.50) from coastal district, Cuttack of Odisha were analyzed and in all 36 native PSB isolates were selected out of which nine (9) isolates with higher P solubilization efficiency were further screened in liquid NBRIP broth supplemented with inorganic phosphates of Calcium, Aluminium, Iron (III) and Iron (II). Identification of PSBs by using TCP usually produces many, but only few are true PSBs. Since soils greatly vary by pH, hence there is a need to identify native and potential isolate with ability to solubilize the phosphates of Al and Fe in acidic soils. Thus the present study aim to establish the P solubilizing efficiency of some PSB strains with inorganic P compounds of Ca, Al, Fe (III) and Fe (II). The native strain CTC12 was observed to have significantly maximum P solubilizing potential with all the inorganic phosphates. Based on the biochemical and molecular characterization (16s rDNA sequencing) isolate CTC12 was identified as Bacillus amyloliquefaciens strain CTC12 (NCBI accession no. KT633845).