ISOLATION AND CHARACTERIZATION OF THERMOPHILIC ACTINOMYCETES WITH EXTRACELLULAR ENZYME AND BIO-SURFACTANT PRODUCTION POTENTIAL FROM THAR DESERT, INDIA

RUCHIKA SHARMA¹*
¹Department of Botany, Jai Narain Vyas University, Jodhpur, Rajasthan
* Corresponding Author : ruchikasharma13@gmail.com

Received : 09-03-2016     Accepted : 30-03-2016     Published : 28-04-2016
Volume : 8     Issue : 4       Pages : 743 - 746
Int J Microbiol Res 8.4 (2016):743-746

Keywords : Thermophilic actinomycetes, extracellular enzymes, bio-surfactant production
Academic Editor : Pritam Omprakash Bhutada, Dr Karan Indra Ostwal, Biplab Kumar Das, Rishikesh Singh, Dr Anil Kumar
Conflict of Interest : None declared
Acknowledgements/Funding : The author is thankful to Department of Science and Technology (Science and Engineering Research Board) for providing fund for research work. My sincere thanks to Dr S Sundaramoorthy (Head) and Dr Praveen Gehlot, Department of Botany, Jai Narain Vyas University, Jodhpur, Rajasthan, India for providing basic research and laboratory facilities. Author also grateful to Prof S.R Rao, Department of Biotechnology, NEHU, Shillong for providing facilities and guidance in research work.
Author Contribution : Sole Authorship

- In the present study thermophilic actinomycetes isolates were isolated from Thar desert of India. Identification of four actinomycetes isolates were done by various morphological and biochemical tests. All the isolates were screened for the presence of extracellular enzyme and bio-surfactant production potential. The results showed that the isolates namely TDI-7, TDI-10, TDI-12, TDI-13 showed the presence of extracellular enzymes and bio-surfactant activity. Isolate TDI-7 and TDI-12 showed the presence of protease production whereas in TDI-10, TDI-12, TDI-13 amylase activity was reported. Lipase activity was found absent in all the isolates. Significant bio-surfactant activity was reported against TDI-13. Further molecular identification of these four isolates were done by partial 16S rRNA gene sequence analysis which showed 95-99 % similarities of references sequences TDI-7 with Streptomyces sp., TDI-10 is with Streptomyces sp., TDI-12 with Streptomyces variabilis strain and TDI-13 with Streptomyces rubiginosus during NCBI blast.