ASSOCIATION OF A NEW 16SrVI SUBGROUP PHYTOPLASMA WITH LITTLE LEAF OF BRINJAL (Solanum melongena L.) GROWN IN KARNATAKA STATE (INDIA)

VANDANA YADAV¹*, S. MAHADEVAKUMAR², G.R. JANARDHANA³, C. AMRUTHAVALLI⁴, M.Y. SREENIVASA^5
1Department of Studies in Microbiology, University of Mysore Manasagangotri, Mysore, 570 006, Karnataka, India
²Molecular Phytodiagnostic Laboratory, Department of Studies in Botany, Manasagangotri, University of Mysore, Mysore, 570 006, Karnataka, India
³Molecular Phytodiagnostic Laboratory, Department of Studies in Botany, Manasagangotri, University of Mysore, Mysore, 570 006, Karnataka, India
⁴Centre for Information Science and Technology, University of Mysore, Manasagangotri, Mysore, 570 006, Karnataka, India
⁵Department of Studies in Microbiology, University of Mysore Manasagangotri, Mysore, 570 006, Karnataka, India
* Corresponding Author : vjshakti@yahoo.co.in

Received : 20-06-2015     Accepted : 21-07-2015     Published : 07-12-2015
Volume : 7     Issue : 6       Pages : 703 - 709
Int J Microbiol Res 7.6 (2015):703-709

Keywords : Little leaf of brinjal, Phytoplasma, Molecular Detection, Molecular Diversity, Karnataka State, 16SrVI-D group, Clover proliferation group, India
Academic Editor : Ranabijuli S., Sinha Bireswar
Conflict of Interest : None declared
Acknowledgements/Funding : The first author would like to thank the Department of Science and Technology, Govt. of India, New Delhi for providing Women Scientist Fellowship (DST No.SR/-WOS-A/ LS-462/2011 Dated. 28/06/2012).
Author Contribution : None declared

Little leaf of brinjal is one of the devastating disease effecting brinjal cultivars worldwide. So far no report is available on molecular detection and characterization of phytoplasma associated with brinjal from South India. Aims and objectives- This study was conducted to detect characterize and group phytoplasma associated with little leaf of brinjal from Karnataka. Materials and methods –Field survey was conducted to collect symptomatic, asymptomatic and healthy samples. Genomic DNA was isolated and subjected to nested PCR with universal primer pairs P1/P7 and R16nF2/R16nR2 respectively for amplification of highly conserved 16SrRNA gene. The amplified products were analyzed on 1.5% agarose gel and the expected nested PCR product of 1250 bp were purified, sequenced in both the directions by Sanger sequencing method and were assembled using Codon Code Aligner software. Multiple sequence alignment was done. Virtual RFLP pattern, restriction map was obtained and phylogenetic tree was constructed. Results and Conclusion - The nBLAST search and phylogenetic analysis of the sequence showed that, all the three samples shared maximum similarity with Clover proliferation (16SrVI) group. The virtual RFLP pattern obtained using iPhyclassifier revealed the association of a new 16SrVI subgroup phytoplasma with one isolate and 16SrVI-D group phytoplasma with other two isolates. This study reports the association of 16SrVI-D and a new sub-group phytoplasma associated with little leaf of brinjal from Karnataka.