S. BHARATHKUMAR1*, P.J. PRAGNYA2, K. JITENDRA3, B. ARCHANA4, M. NIHARIKA5, N. NUPUR6, S. SULAGNA7, M. SOUMYA8, P.M. DURGA9, J.N. REDDY10
1Department Crop Improvement Division, ICAR-Central Rice Research Institute (CRRI), Cuttack, Odisha-753 006, India
2Department Crop Improvement Division, ICAR-Central Rice Research Institute (CRRI), Cuttack, Odisha-753 006, India
3Department Crop Improvement Division, ICAR-Central Rice Research Institute (CRRI), Cuttack, Odisha-753 006, India
4Department Crop Improvement Division, ICAR-Central Rice Research Institute (CRRI), Cuttack, Odisha-753 006, India
5Department Crop Improvement Division, ICAR-Central Rice Research Institute (CRRI), Cuttack, Odisha-753 006, India
6Department Crop Improvement Division, ICAR-Central Rice Research Institute (CRRI), Cuttack, Odisha-753 006, India
7Department Crop Improvement Division, ICAR-Central Rice Research Institute (CRRI), Cuttack, Odisha-753 006, India
8Department Crop Improvement Division, ICAR-Central Rice Research Institute (CRRI), Cuttack, Odisha-753 006, India
9Department Crop Improvement Division, ICAR-Central Rice Research Institute (CRRI), Cuttack, Odisha-753 006, India
10Department Crop Improvement Division, ICAR-Central Rice Research Institute (CRRI), Cuttack, Odisha-753 006, India
* Corresponding Author : bharathkumar76@gmail.com
Received : 12-08-2015 Accepted : 28-10-2015 Published : 15-11-2015
Volume : 7 Issue : 2 Pages : 177 - 179
Genetics 7.2 (2015):177-179
Keywords : Salinity, Submergence, Rice landraces, Microsatellite (SSR) markers, Sub1 and Saltol locus.
Academic Editor : Dr Nasser Samadi,Zaveri Anurag D,Dr. Rajinder Kaur, M. Santoshi Kumari,Anil Kumar, Fidyan A. Alkhowaiter,Dr. Anjali Singh, Dr. Mitesh Kumar Dwivedi,Tekcham Dinesh Singh,Tianxu Han, I S Chakrapani,Dr. Gautami Das,Khushdeep Bandesh, Abdul Majid, Dr. Manjush
Conflict of Interest : None declared
Acknowledgements/Funding : This work was funded by Department of Biotechnology (DBT), INDIA. We sincerely thank the Director, CRRI for providing facilities to conduct this experiment.
Author Contribution : None declared
In coastal regions, both salinity and submergence stress alternatively or simultaneously become severe constrain to the rice cultivation by climate changes. This situation necessitates the search of combined tolerance to salinity and submergence stress from the source of germplasms lines. In this study, eight rice landraces collected from coastal region of eastern India were screened for salinity and submergence tolerance at phenotypic and genotypic level. In salinity screening, six rice landraces (Rupsal, Marishal, Polai, Talmugra, Kamini and Raspangar) were identified as salinity tolerant. In PCR screening using SSR markers located in Saltol locus, Pokkali alleles (tolerant check) for AP3206 and RM3412 markers and non-Pokkali alleles for RM8094 marker were detected in salinity tolerant genotypes. The results of RM8094 coincided with that of salinity screening. Moreover, none of the landraces showed significant tolerance as FR13A (tolerant check) in submergence screening. In PCR screening using SSR and gene-specific markers of Sub1 locus, FR13A alleles were documented in intolerant genotypes also. However, the results of Sub1BC2 (InDel) marker coincided with that of submergence screening.