IN VITRO ADHERENCE OF COAGULASE-NEGATIVE Staphylococcus STRAINS IN CATHETERS OF DIFFERENT MATERIAL COMPOSITION

V.B. BINATTI¹*, P.M.A. PEREIRA², B.S.P. RIBEIRO³, E.A. RIBEIRO DE CASTRO⁴, A.M. PEREIRA⁵, A. BELLO⁶, R.S. LEÃO⁷, E.A. MARQUES⁸, J.A.A. PEREIRA^9
1Departamento de Microbiologia, Imunologia e Parasitologia. Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro.
²Departamento de Microbiologia, Imunologia e Parasitologia. Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro.
³Departamento de Microbiologia, Imunologia e Parasitologia. Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro.
⁴Hospital Universitário Pedro Ernesto, Universidade do Estado do Rio de Janeiro.
⁵Hospital Universitário Pedro Ernesto, Universidade do Estado do Rio de Janeiro.
⁶Departamento de Microbiologia, Imunologia e Parasitologia. Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro.
⁷Departamento de Microbiologia, Imunologia e Parasitologia. Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro.
⁸Departamento de Microbiologia, Imunologia e Parasitologia. Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro.
⁹Departamento de Microbiologia, Imunologia e Parasitologia. Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro.
* Corresponding Author : vanessabinatti@globo.com

Received : 10-09-2015     Accepted : 03-10-2015     Published : 12-10-2015
Volume : 7     Issue : 4       Pages : 671 - 675
Int J Microbiol Res 7.4 (2015):671-675

Keywords : Coagulase-negative Staphylococci, Vascular catheter, Bacterial adherence
Academic Editor : Anas Rashid, Maryam Yasemi, Amita Shobha Rao, Begum F, Agnieszka Bogut, Judit Szabo, Noureldaim Elnoman Elbadawi, Amany El-sharif, Hiba Siddig Ibrahim Mustafa, Thura A. Kadhim
Conflict of Interest : None declared

Coagulase-negative staphylococci (CoNS) are a major cause of infections associated with indwelling medical devices. Strains of CoNS produce slime and form biofilms on polymer surfaces, which is associated with their pathogenicity. This study evaluated the correlation between adherence of CoNS and composition of vascular catheters composed of polyurethane and silicone, by the phenotypic analysis of slime production, the presence of icaAD and atlE genes, and by comparing the relationship between oxacillin-resistant (MRS) and oxacillin-susceptible (MSS) CoNS and biofilm formation. All MRS isolates included in this study demonstrated the ability to form biofilms but on the other hand, only 50% of MSS were capable of forming biofilms. All CoNS demonstrated degrees of adherence using different materials (polyurethane and silicone). The results of this study suggest a strong correlation between slime production and the level of resistance to oxacillin, since 100% of MSS strains did not produce slime and 90% of MRS strains were slime producers. Two particular hospital samples were notable due to their high MICs (≥ 128 mg/mL), with both demonstrating the ability to produce slime and the presence of the icaAD gene. These findings emphasize the choice of material of the catheter and the required care to be taken at the time of insertion and maintenance to avoid contamination with CoNS. Our results highlight that continuous training for correct handling measures is necessary to prevent intravascular as well as peripheral catheter infections on the hospital ward.