ISOLATION AND CHARACTERIZATION OF Proteus spp. FROM DOMESTIC DISPOSAL EXPRESSING EXTRACHROMOSOAL EXTENDED SPECTRUM β-LACTAMASE TRAIT

A.A. KHARAT¹, S. BAWALE², H. OMAN³, D.G. KADAM⁴, A.S. KHARAT⁵*
¹Department of Microbiology, DBF Dayanand College of Arts & Science, Solapur - 413 002, MS, India.
²Department of Biotechnology, Dr. Babasaheb Ambedkar Marathwada University, Sub Campus, Osmanabad - 413 501, MS, India.
³Department of Biotechnology, Dr. Babasaheb Ambedkar Marathwada University, Sub Campus, Osmanabad - 413 501, MS, India.
⁴Department of Microbiology, DBF Dayanand College of Arts & Science, Solapur - 413 002, MS, India.
⁵Department of Biotechnology, Dr. Babasaheb Ambedkar Marathwada University, Sub Campus, Osmanabad - 413 501, MS, India.
* Corresponding Author : arunkharat2007@gmail.com

Received : 15-08-2015     Accepted : 22-09-2015     Published : 24-09-2015
Volume : 7     Issue : 1       Pages : 170 - 176
Genetics 7.1 (2015):170-176

Keywords : Domestic disposal, Enterobacteriaceae, Disc diffusion, ESBL, plasmid curing
Academic Editor : Anas Rashid, Jayanta Mistry, Jayanta Mistry, Huxley Mae Makonde, Chandani T. Desai, Tianxu Han, Tairon Pannunzio Dias E Silva, Nabin N Munankarmi
Conflict of Interest : None declared

From a long time waterborne diseases have been major concern to living being. Handful countries have successfully implemented recycling of water. Recycling of water is under consideration in India. Indian rural region is yet to establish domestic disposal and sewage treatment plant. Number of villages due to insufficient rain depends largely on bore well water, a type of hard water. Pumped water off the bore well is coming from underground depth of which varies from village/town to town. We have isolated and characterized an enteric bacterium from domestic disposal, which exhibits Extended Spectrum β-Lactamase (ESBL) trait. The domestic disposal was enriched with triple strength MacConkeys broth. Cultural and biochemical characterization of various isolates was carried as per Bergeys Manual of Systematic bacteriology. Based on Morphological, Cultural, and Biochemical characters these isolates were Citrobacter spp, Proteus rettgeri, Proteus morganii, Providencia spp, Escherichia coli, Klebsiella spp and Hafnia alvei. All of these isolates were screened to determine resistance to third generation cephalosporins such as; Cefotaxime (CTX), Cefepime (CPM) and Ceftazidime (CAZ). Amongst ten isolates, AKA10 strain of Proteus spp, exhibited resistance to β-lactms as Penicillin, Ampicillin, Oxacillin Coxacillin and third generation cephalosporins. Phenotypic classification indicated that ESBL trait belongs to OXA-48 type. Genetic manipulations A. Plasmid curing and B. plasmid transformation indicated that ESBL trait is extrachromosomal and contains a broad host range replicon, perpetuating not only in Proteus but also in E. coli. Implications of ESBL trait in domestic disposal are discussed.