NEONATAL SEPSIS - A PROSPECTIVE STUDY FROM A TERTIARY CARE HOSPITAL OF NORTH DELHI

M. SHARMA¹, S. JAIN², N. SHREE³*, D. BHAGWANI⁴, M. KUMAR^5
1Department of Microbiology, North Delhi Municipal Corporation Medical College & Hindu Rao Hospital, Delhi - 110007, India.
²Department of Microbiology, North Delhi Municipal Corporation Medical College & Hindu Rao Hospital, Delhi - 110007, India.
³Department of Microbiology, North Delhi Municipal Corporation Medical College & Hindu Rao Hospital, Delhi - 110007, India.
⁴Department of Pediatrics, North Delhi Municipal Corporation medical College & Hindu Rao Hospital, Delhi - 110007, India.
⁵Department of Microbiology, North Delhi Municipal Corporation Medical College & Hindu Rao Hospital, Delhi - 110007, India.
* Corresponding Author : neetushree@gmail.com

Received : 30-04-2015     Accepted : 07-09-2015     Published : 10-09-2015
Volume : 7     Issue : 3       Pages : 644 - 647
Int J Microbiol Res 7.3 (2015):644-647

Keywords : Neonatal Sepsis, Multi Drug Resistance, Antibiogram
Academic Editor : Dr Moumita Sardar
Conflict of Interest : None declared

Background: Neonatal sepsis is a common cause of morbidity and mortality among newborns in developing world. Most cases are identified based on the clinical manifestations presenting in early or late neonatal period. An early treatment and the appropriate use of antibiotics would minimize the risk of morbidity and mortality in neonatal sepsis. Objective: To study demographical, bacteriological, and laboratory profile of neonates presenting with clinically suspected sepsis based on predefined clinical criteria. Study Design: A prospective study involving a total of 200 neonates with clinical suspicion of NS, between November 2013 to may 2014 admitted at NICU at NDMC and Hindu Rao Hospital, North Delhi. Materials and Methods: Blood cultures were done from 200 neonates admitted in NICU suspected to have sepsis. Samples were incubated in the Bactec 9120. Isolates identified and AST performed by VITEK 2C. The results were evaluated for demographical, bacteriological, and laboratory profile. Results: Out of 200 neonates, 119(59.5%) were early onset sepsis and 81 (40.5%) neonates with late onset sepsis. Male constituted 61% (122) and females 39 % (78), ratio being 1.6:1. Septic screen was positive in 72% (144) neonates and culture positive in 23% (46) neonates. Of the organisms cultured, S.aureus 39.1% (18) was predominant pathogen in both EONS and LONS. Other isolates include Klebsiella sp., E. coli, P. aeruginosa and Gr. B Streptococcus. Isolates showed variable resistance towards various antimicrobial agents. Overall, Imipenem, vancomycin and linezolid were the most effective antimicrobial agents comparatively with statistically significant difference in sensitivity but, these should not be used indiscriminately and kept as reserved options to prevent emergence of MDR strains and treatment failure. Conclusion: Knowledge of locally prevalent microorganism and antibiogram pattern is important in the management of neonatal sepsis. GPC were the main cause of neonatal sepsis. The data must be periodically reviewed and antibiotic policy revised accordingly.