OCCURRENCE AND MICROBIAL PROFILE OF BLOOD STREAM INFECTION IN TERTIARY CARE HOSPITAL INDIA

C. VYAWAHARE¹, S.V. JADHAV²*, R.N. MISRA³, N. GANDHAM⁴, K. ANGADI⁵, N. GUPTA^6
1Department of Microbiology, Dr. D.Y. Patil Medical College, Hospital and Research Centre (Dr. D.Y. Patil University) Pune - 411 018, MS, India.
²Department of Microbiology, Dr. D.Y. Patil Medical College, Hospital and Research Centre (Dr. D.Y. Patil University) Pune - 411 018, MS, India.
³Department of Microbiology, Dr. D.Y. Patil Medical College, Hospital and Research Centre (Dr. D.Y. Patil University) Pune - 411 018, MS, India.
⁴Department of Microbiology, Dr. D.Y. Patil Medical College, Hospital and Research Centre (Dr. D.Y. Patil University) Pune - 411 018, MS, India.
⁵Department of Microbiology, Dr. D.Y. Patil Medical College, Hospital and Research Centre (Dr. D.Y. Patil University) Pune - 411 018, MS, India.
⁶Department of Microbiology, Dr. D.Y. Patil Medical College, Hospital and Research Centre (Dr. D.Y. Patil University) Pune - 411 018, MS, India.
* Corresponding Author : patilsv78@gmail.com

Received : 29-06-2015     Accepted : 30-07-2015     Published : 06-08-2015
Volume : 7     Issue : 2       Pages : 631 - 635
Int J Microbiol Res 7.2 (2015):631-635

Keywords : Blood stream infection, multidrug resistance, ESBL, MBL, Inducible clindamycin producer Staphylococcus aureus
Academic Editor : Bhat Asha V., Dr. Ramaiah Pushpamala, Pateel Praveen S., Hubballi Jagadeesh G, Arya Varun, Poonam Sharma, Mann Akshita, Viswanath Lekha, N. Gayathri, Bhagya Seela S, Pateel Praveen S., Chitra P
Conflict of Interest : None declared

Background: The epidemiology of blood stream infection (BSI) is constantly changing. BSIs are associated with the syndrome requiring admission to intensive care unit (ICU) such as sepsis and septic shock and remains one of the most important causes of morbidity and mortality. BSI prolongs patients stay in an ICU and in hospital leads to increased health care expenses. Methods: This laboratory based retrospective study was conducted for a period of two years (January 2011 to December 2012) in a 1470 bed tertiary care hospital in India. A total of 2999 samples were analyzed from the hospitalized patients for whom blood culture were routinely processed in the department of Microbiology Laboratory. Results: The present retrospective study demonstrated 966 (32.21%) culture positivity. Of the total culture positive episodes, 374 (38.81%) were GNP and 573 (59.31%) were GPP while 19 (1.96%) were Candida spp.. Of the total 573 GPP, 258 (45.02%) were MSSA while 228 (39.79%) were MRSA. Among 486 S. aureus strains isolated from BSIs, 129 (26.54%) strains were inducible clindamycin producers of which 99 (76.74%) strains were isolated from patients admitted in various ICU. All S. aureus strains were susceptible to linezoilid and tigecycline and vancomycin. Present study revealed that 431 (44.61%) positive culture episodes were from patients admitted in various ICU of which 239 (55.45%) were GNP and 192 (44.54%) were GPP. Maximum patients were from MICU 162 (37.58%) and NICU 153 (35.49%) followed by medicine ward and orthopedic ward. Conclusion: The surveillance of BSI pathogen in a hospital is important in monitoring the spectrum of microorganism that invades the blood stream and to improve efficacy of empirical treatment protocols. It helps to clinicians to recognize the emerging pathogens that may be threat to the community. Collaboration between physicians, clinical microbiologists and infectious disease consultants should produce significant positive outcome.