COMPARATIVE ANALYSIS OF INDUCIBLE CLINDAMYCIN RESISTANCE AMONG COMMUNITY AND HOSPITAL ASSOCIATED Staphylococcus aureus INFECTION IN TERTIARY CARE HOSPITAL MAHARASHTRA INDIA

D. DESAI¹*, R.N. MISRA², K. YEDIDI³, N.R. GANDHAM⁴, K.M. ANGADI⁵, S.V. JADHAV^6
1Dr. D.Y. Patil Medical College, Hospital and Research Centre (Dr. D.Y. Patil University), Pimpri, Pune- 411 018, MS, India.
²Dr. D.Y. Patil Medical College, Hospital and Research Centre (Dr. D.Y. Patil University), Pimpri, Pune- 411 018, MS, India.
³Dr. D.Y. Patil Medical College, Hospital and Research Centre (Dr. D.Y. Patil University), Pimpri, Pune- 411 018, MS, India.
⁴Dr. D.Y. Patil Medical College, Hospital and Research Centre (Dr. D.Y. Patil University), Pimpri, Pune- 411 018, MS, India.
⁵Dr. D.Y. Patil Medical College, Hospital and Research Centre (Dr. D.Y. Patil University), Pimpri, Pune- 411 018, MS, India.
⁶Dr. D.Y. Patil Medical College, Hospital and Research Centre (Dr. D.Y. Patil University), Pimpri, Pune- 411 018, MS, India.
* Corresponding Author : desaidipalee15@gmail.com

Received : 31-03-2015     Accepted : 28-05-2015     Published : 03-08-2015
Volume : 7     Issue : 2       Pages : 623 - 626
Int J Microbiol Res 7.2 (2015):623-626

Keywords : inducible MLS B resistant, S. aureus, HA-MRSA, CA-MRSA
Academic Editor : Alexander Pedroza-gonzalez, Dr. Vivek Jadhav, Lalitha K, Dr Sudhir, Madhusudan M, Desai Vela D
Conflict of Interest : None declared
Acknowledgements/Funding : We would like to extend our thanks to STS 2014, Indian Council of Medical Research (ICMR) New Delhi. We also thankful to members of the D.Y. Patil University, Pune and Chairmen for their help and support. We acknowledge technical assistance provided by Mi

Introduction: Increasing frequency of Staphylococcus aureus (S. aureus) infections and changing patterns in antimicrobial resistance have led to concern in the use of macrolide lincosamide streptogramin type B (MLSB) antibiotics to treat such infections. However, their widespread use has led to an increase in the number of S. aureus strains resistant to MLSB antibiotics. Clindamycin is an appealing option because of its proven efficacy, safety and convenience of parental and oral administration in patients. The possibility of inducible resistance to clindamycin is a concern and has left very few therapeutic options for clinicians. Material and Methods: A total of 100 S. aureus strains were isolated from various clinical samples and were tested for antimicrobial susceptibility testing by Kirby-Bauer disc diffusion method and detection of iducible MLSB were detected by double disc diffusion D-zone test method. Results: Of the total 100 S. aureus strains all were sensitive to vancomycin and linezolid while 50% were resistant to Oxacillin. Of the total 100 S. aureus 68 % were resistant to erythromycin of that 30 were inducible clindamycin resistant showed D-zone test positive and 90% isolates were isolated from pyogenic infections. Of the total 30 % inducible clindamycin positive isolated, 10 (33.33%) were methicillin resistant S. aureus (MRSA) while 20 (66.66%) were mithicillin sensitive S. aureus (MSSA). Of the total inducible clindamycin positive isolates 7 (70%) isolates were HA-MRSA and 3(30%) were CA-MRSA. Conclusion: Double disc diffusion D- zone test should be used as a mandatory method in routine disc diffusion testing to detect inducible clindamycin resistance.