ISOLATION AND CHARACTERIZAION OF HYDROLYTIC ENZYME PRODUCING HALOPHILIC BACTERIA Salinicoccus roseus FROM OKHA

R.K. RAVI¹*, P. PATEL^2
1Department of Biotechnology, Shree M. & N. Virani Science College, Rajkot - 360 005, Gujarat, India.
²Department of Biotechnology, Christ College, Rajkot - 360 005, Gujarat, India.
* Corresponding Author : raviranjan@vsc.edu.in

Received : 15-01-2015     Accepted : 18-05-2015     Published : 03-08-2015
Volume : 7     Issue : 2       Pages : 616 - 622
Int J Microbiol Res 7.2 (2015):616-622

Keywords : Phylogenesis, Halophiles, Hydrolytic enzymes, Amylase, Salinicoccus roseus
Academic Editor : Anil Kumar Singh
Conflict of Interest : None declared

Halophilic bacteria were isolated and purified from marine water of Okha region of Gujarat, India and further subjected to screen for extracellular hydrolytic enzyme production. Two strains (R1 & R2) showed extracellular hydrolytic enzyme activities, such as amylases, proteases, lipases and gelatinase, where as optimum activity observed with amylase. They were characterized on the basis of morphology, physiology and biochemical tests. The phylogenetic tree revealed that the strains R1 & R2 fit in to an evolutionary cluster comprising members of Salinicoccus roseus with 99% similarity. 16S rDNA sequence was submitted at NCBI and named as Salinicoccus roseus strain rvscokh1 & rsk1 respectively. The DNA G+C content rvscokh1 & rsk1 was 55.6 & 55.7 mol% respectively. The GenBank accession number of the 16S rDNA sequences of strain rvscokh1 & rsk1 is HQ190916 & HQ258884 respectively. Salinicoccus roseus is gram positive coccus bacteria. Strain rvscokh1 & rsk1 sustain to 10% and 6% NaCl concentration respectively while optimum pH was 7.4 for growth. Optimum amylase activity was found at temperature 37°C, pH 8 and 5% salt concentration for both the strains reveals that these strains can produce potentially industrially important enzymes.