FREQUENCY DISTRIBUTION OF Plasmodium falciparum MSP-2 ALLELE AND ASSOCIATION WITH CLINICAL MANIFESTATIONS AND DEMOGRAPHIC FACTORS IN JAYAPURA MUNICIPAL, PAPUA PROVINCE, INDONESIA

Y. SORONTOU¹*, A. PAKPAHAN^2
1Ministry of Health, Polytechnic of Health Jayapura, Papua Province, Indonesia 99100.
²Department of Biology, Faculty of Medicine and Faculty of Dentistry, Trisakti University, Indonesia 11440.
* Corresponding Author : yohanna.sorontou@gmail.com

Received : 20-03-2015     Accepted : 28-04-2015     Published : 15-06-2015
Volume : 7     Issue : 1       Pages : 143 - 151
Int J Parasitol Res 7.1 (2015):143-151

Keywords : Clinical manifestation, Malaria severity, msp2 allele, Plasmodium falciparum
Academic Editor : Dr. ML. Edy Parwanto, MBiomed., Dr. Kusmardi, MS., Prof. Dr. Shirly Kumala, Apt, Dr Kempaiah Rayavara, Dr. Paramjit Kaur, Dr. Navpreet Kaur
Conflict of Interest : The authors have declared that they have no competing interest.
Acknowledgements/Funding : We gratefully thank the Director of Polytechnic of Health Jayapura, Staffs in Jayapura and Abepura and Jayapura General Hospitals, and Community Health Center in Skow, Jayapura Municipalities Papua Province, Indonesia. We are also grateful Dean, Dr. Pilar

The merozoite surface protein-2 (msp2) of Plasmodium falciparum is a polymorphic glycoprotein expressed on the surface membrane of the merozoite, and plays major role in the erythrocyte invasion. Certain msp2 allele has been associated with malaria severity. The present study aims to explore the association between the msp2 allele with the clinical manifestations and demographic factors in Papua, Indonesia. Malaria asymptomatic cases were obtained from the community health centers in Koya and Skow villages, whereas symptomatic cases were obtained from Jayapura and Abepura General Hospitals. A total of 152 blood samples, 51 asymptomatic and 101 symptomatic subjects, were analyzed by microscopic examination and PCR amplification. The results indicated that the IC1/3D7 allele was the dominant allele in both groups, with the frequency distribution in asymptomatic malaria was 52.9% for IC1/3D7 allele, 19.6% for FC27 allele, and 27.5% for mix of IC1/3D7 and FC27 alleles. In symptomatic malaria cases, the frequency distribution was 50.5% for IC1/3D7allele, 22.8% for FC27 allele, and 26.7% for mix of IC1/3D7 and FC27 alleles. Similarly in the symptomatic subjects, IC1/3D7 allele was shown to be more frequently detected than FC27 allele in both level of parasite density. Statistical analysis using Pearson Chi-square showed that there is no significant correlation between the P. falciparum msp2 allele and clinical manifestation, parasite density, age and gender. FC27 allele seemed to be increased in frequency in severe malaria and females subjects.