QUANTITATIVE ANALYSIS OF BIOFILM FORMED BY SALMONELLA ENTERITIDIS BY USING DIFFERENT MEDIA AND SUBSTRATES

S.S. CHOUGULE¹, M. CHANDRA²*, S. THAKUR³, D. NARANG⁴, G. KAUR⁵, N.S. SHARMA^6
1Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana -141 004, Punjab, India
²Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana -141 004, Punjab, India
³Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana -141 004, Punjab, India
⁴Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana -141 004, Punjab, India
⁵Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana -141 004, Punjab, India
⁶Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana -141 004, Punjab, India
* Corresponding Author : drmuditchandra@rediffmail.com

Received : 10-03-2015     Accepted : 09-04-2015     Published : 21-04-2015
Volume : 7     Issue : 1       Pages : 610 - 612
Int J Microbiol Res 7.1 (2015):610-612

Keywords : Salmonella, Biofilm, LB, TSB, Chitin, Plastic, Glass wool, Medium
Academic Editor : Dr Sashi Kanta Dash, Agnes Wiedemann
Conflict of Interest : Author declares that, there is no conflict of interest.
Acknowledgements/Funding : The authors are grateful to The Department of Biotechnology (DBT) India for the financial support. The authors are also thankful to the Director Research, GADVASU for providing the necessary laboratory facilities.

Salmonella Enteritidis biofilm was formed successfully in vitro by using five different media and substrate combination. The total biomass of the biofilm formed using different media and substrate varied considerably. The best biofilm was observed when the bacterium was grown in TSB in combination with 1% Chitin (4.749±0.1), while the least biofilm was formed in LB in combination with 1% Glass wool (1.539±0.02). The average of all the media irrespective of substrates revealed that in RPV maximum biofilm (4.068±0.144) was formed whereas, the least was formed in LB.