<i>IN VITRO</i> ORGANOGENESIS OF NAGA CHILLI (<i>Bhot jolokia</i>) VIA LEAF DERIVED CALLUS CULTURE

GEETASHREE B.¹, HEMANTA K.G.², PRATAP J.H.^3
1Division of Biotechnology, Defence Research Laboratory (DRL), Tezpur- 784 001, Assam, India.
²Division of Biotechnology, Defence Research Laboratory (DRL), Tezpur- 784 001, Assam, India.
³Department of Biotechnology, Gauhati University, Guwahati- 781 014, Assam, India.

Received : 02-01-2014     Accepted : 06-02-2014     Published : 06-03-2014
Volume : 3     Issue : 1       Pages : 35 - 38
World Res J Agr Biotechnol 3.1 (2014):35-38

Conflict of Interest : None declared

This communication brings into focus for the first time, a complete standardized protocol pertaining <i>in vitro</i> micropropagation in Naga Chilli (Bhot Jolokia) via indirect organogenesis from leaf explants under the influence of different plant growth hormones. Maximum number of callus induction and highest number of multiple shoots initiation were recorded under the influence of Benzyl Amino Purine (BAP) at the concentration of 45 µM/L, along with 2,4- Dichlorophenoxy Acetic Acid (2,4-D) (3.5 µM/L) and Silver Nitrate (AgNO₃) at the rate of 35µM/L. AgNO₃ ­(34 µM/L) and Gibberellic Acid (GA₃) (28 µM/L) were reported to be the optimum concentrations for <i>in vitro</i> flower induction and fruit development. <i>In vitro</i> regenerated shoots were cultured in Murashige and Skoog (MS) medium fortified with IBA (3.5 µM/L) and 2,4-D (4.5 µM/L) for maximum root initiation. Healthy rooted plantlets were transferred to potting substrate containing half strength MS medium enhanced with soil and vermicompost in equal ratio (1:1) for hardening in the poly house. 78.65% of total acclimatized plants were transferred to the main field.