STIMULATORY EFFECT OF FASTING AND DIABETES MELLITUS ON SALIVARY ALPHA AMYLASE LEVEL

NWANGWA E.¹, EKHOYE E.^2
1Department of Human Physiology, P.M.B 001,Delta State University, Abraka, Nigeria.
²Department of Human Physiology, P.M.B 001,Delta State University, Abraka, Nigeria.

Received : 06-11-2013     Accepted : 03-12-2013     Published : 28-12-2013
Volume : 2     Issue : 1       Pages : 4 - 7
World Res J Diabetes 2.1 (2013):4-7

The objective of this study was to determine the effect of fasting and diabetes mellitus on salivary alpha amylase level. Eighty female subjects comprising of healthy volunteers and patients suffering from diabetes at Eku Baptist hospital were randomly selected for this study. They were divided into eight groups of ten subjects each. Group A was made up of Normal subjects, Group B: 12 hours fasting Subjects, Group C: 18 hours fasting Subjects, Group D:24 hours fasting Subjects, Group E: Four hours after twenty four hour fasting Subjects, Group F: Diabetic subjects, Group G: Fasting diabetic subjects. After the experiment, saliva was collected and saliva alpha amylase assay was carried out. Statistical significance between normal subjects (control) and experimental groups was done using Students t-test and One way Analysis of Variance (ANOVA), and a level of p<0.05 was considered significant. Saliva alpha amylase significantly (p<0.05) increased in the subjects in a fasting duration dependent manner. The saliva alpha amylase level reduced after fasting. Also in this study, diabetes mellitus significantly (p<0.05) increased the saliva alpha amylase level. Therefore, this study shows that saliva alpha amylase increases during period of fasting and diabetes mellitus condition.