EROSION KINETICS OF PROTEIN COACERVATE ENTRAPPING MOSQUITO LARVICIDE CYCLOHEXIMIDE (CHX) AT VARIOUS PH

CHANCHAL A.¹, BOHIDAR H.B.², VERMA A.K.^3
1Department of Zoology, K.M. College, University of Delhi, New Delhi-110007, Delhi, India.
²School of Physical Sciences, Jawaharlal Nehru University, New Delhi-110067, Delhi, India.
³Department of Zoology, K.M. College, University of Delhi, New Delhi-110007, Delhi, India.

Received : 21-11-2013     Accepted : 18-12-2013     Published : 26-12-2013
Volume : 1     Issue : 2       Pages : 38 - 42
World Res J Pharmaceut Res 1.2 (2013):38-42

A homogenous solution of oppositely charged macromolecules may undergo liquid-liquid phase separation through associative interactions, it can then give rise to a polymer rich dense phase, called coacervates. The dissolution kinetics and the release profile of the inter-molecular aggregates and self-assembled gelatin coacervate material entrapping model larvicide Cycloheximide (CHX), at various pH values were assessed. Sustained release of CHX was observed and read by UV spectrophotometer at 250nm at various time points. The dissolution/erosion kinetics revealed that the coacervate sustained longer in Phosphate Buffer Saline pH-7.4, than at acidic pH-5 or alkaline pH-8 and doubled distilled water. Efficacy studies were done to assess the susceptibility of the mosquito larvae to the coacervates material and CHX over a period of 100 hours. The probit values of the recorded mortality were plotted against log concentration and LC50 and LC99 values were determined. The high entrapment efficiency of the coacervate when compared to other systems makes it a good candidate for bio-pharmaceutics, not only human therapeutics but also in pest control management.