ISOLATION AND CHARACTERIZATION OF INDIGENOUS CELLULASE PRODUCING Trichoderma SPP. FROM BANGLADESH

CHOWDHURY A.1, AZAM M.S.2, FAKRUDDIN M.D.3, HOSSAIN M.N.4, KHANDAKER R.M.5, HOQUE M.M.6, MONZUR M.A.7
1Industrial Microbiology Laboratory, Institute of Food Science and Technology (IFST), BCSIR, Bangladesh.
2Department of Food Engineering and Tea Technology, Shahjalal University of Science & Technology, Sylhet, Bangladesh.
3Industrial Microbiology Laboratory, Institute of Food Science and Technology (IFST), BCSIR, Bangladesh.
4Industrial Microbiology Laboratory, Institute of Food Science and Technology (IFST), BCSIR, Bangladesh.
5Industrial Microbiology Laboratory, Institute of Food Science and Technology (IFST), BCSIR, Bangladesh.
6Department of Food Engineering and Tea Technology, Shahjalal University of Science & Technology, Sylhet, Bangladesh.
7Industrial Microbiology Laboratory, Institute of Food Science and Technology (IFST), BCSIR, Bangladesh.

Received : 31-10-2013     Accepted : 03-12-2013     Published : 21-12-2013
Volume : 1     Issue : 2       Pages : 32 - 35
World Res J Biotechnol 1.2 (2013):32-35

Cite - MLA : CHOWDHURY A., et al "ISOLATION AND CHARACTERIZATION OF INDIGENOUS CELLULASE PRODUCING Trichoderma SPP. FROM BANGLADESH." World Research Journal of Biotechnology 1.2 (2013):32-35.

Cite - APA : CHOWDHURY A., AZAM M.S., FAKRUDDIN M.D., HOSSAIN M.N., KHANDAKER R.M., HOQUE M.M., MONZUR M.A. (2013). ISOLATION AND CHARACTERIZATION OF INDIGENOUS CELLULASE PRODUCING Trichoderma SPP. FROM BANGLADESH. World Research Journal of Biotechnology, 1 (2), 32-35.

Cite - Chicago : CHOWDHURY A., AZAM M.S., FAKRUDDIN M.D., HOSSAIN M.N., KHANDAKER R.M., HOQUE M.M., and MONZUR M.A. "ISOLATION AND CHARACTERIZATION OF INDIGENOUS CELLULASE PRODUCING Trichoderma SPP. FROM BANGLADESH." World Research Journal of Biotechnology 1, no. 2 (2013):32-35.

Copyright : © 2013, CHOWDHURY A., et al, Published by Bioinfo Publications. This is an subscription based article distributed under the terms of the Creative Commons Attribution License, in which, you may not use the material for commercial purposes, you may not distribute the modified material.

Abstract

Organisms producing cellulase play a key role in the recycling of cellulose, and thereby maintain the carbon cycle on earth. Members of the genus Trichoderma are well known to secrete active cellulase into the culture broth. Present study aims to isolate and characterize indigenous cellulytic Trichoderma spp. from Bangladesh. In this study, decaying wood samples were screened for the isolation of Trichoderma spp. capable of producing cellulase using CMC (Carboxymethyl cellulose) as a substrate. Cellulase production by the isolates was examined at different conditions. Ten filamentous fungi capable of producing cellulase were isolated. Based on morphological characteristics they were identified as member of Trichoderma sp. The results showed that all the selected isolates produced detectable quantities of cellulases on plate screening medium. The effect of various physical parameters such as temperature, pH, rotation, incubation time etc. on enzyme production of the isolates were determined. All the strain exhibited over all good cellulytic activity after 72 hours of incubation at 30± 2ºC at pH 4.0. Rotation has shown to affect enzyme productivity and productivity was higher at RPM 100. Cellulase productivity can be improved further through strain mutation and optimization of the fermentation process.