DECOLORIZATION OF A RECALCITRANT ORGANIC HYDROCARBON (MELANOIDIN) BY A POTENTIAL THERMOTOLERANT CONSORTIUM OF BACTERIA, Pediococcus sp. AND YEAST, Candida sp.

TIWARI S.¹, GAUR R.², RAI P.^3
1Department of Microbiology (Centre of Excellence), Dr. Ram Manohar Lohia Avadh University, Faizabad- 224 001, UP, India.
²Department of Microbiology (Centre of Excellence), Dr. Ram Manohar Lohia Avadh University, Faizabad- 224 001, UP, India.
³Department of Microbiology (Centre of Excellence), Dr. Ram Manohar Lohia Avadh University, Faizabad- 224 001, UP, India.

Received : 25-03-2012     Accepted : 25-07-2013     Published : 19-08-2013
Volume : 1     Issue : 1       Pages : 8 - 15
World Res J Cell Biol 1.1 (2013):8-15

The aim of this study was to isolate microorganisms capable of decolourizing distillery spentwash. A consortium comprising of one bacterium and one yeast was selected on the basis of rapid effluent decolourization, which exhibited 82±1.5% decolourization within 24 hrs. when incubated at 45°C under static condition in effluent supplemented with 0.1%, glucose; 0.1%, peptone; 0.05%, MgSO₄; 0.01%. The cultures were identified as Pediococcus acidilactici by 16S rDNA analysis and Candida tropicalis on the basis of phenotypic level. It is the first time when thermotolerant melanoidin decolourizing consortium (Pediococcus acidilactici and Candida tropicalis) isolated from distillery soil was capable to decolourizing melanoidin pigment of distillery effluent. Hence, it was observed that consortium has the ability to degrade the spentwash efficiently. This investigation could be an approach towards control of environmental pollution and health hazards of humans in and around the distillery site.