IMPACT OF <i>Schistosoma mansoni</i> CO-INFECTION WITH CHRONIC HEPATITIS C ON SERUM LEVELS OF INTERLEUKIN-10, OXIDATIVE STRESS AND ANTI-OXIDANT PROFILE

EL-KHOLY E.I.¹, EL-BENDARY M.M.², METWALI A.A.³, NOURELDIN M.S.⁴, EL-BESHBISHI S.N.⁵, EL-SAMANOUDY A.Z.⁶, SALEM D.A.^7
1Medical Parasitology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
²Tropical Medicine & Hepatology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
³Internal Medicine Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
⁴Medical Parasitology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
⁵Medical Parasitology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
⁶Medical Biochemistry Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
⁷Medical Parasitology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt.

Received : 12-05-2012     Accepted : 01-07-2013     Published : 06-08-2013
Volume : 1     Issue : 1       Pages : 1 - 5
World Res J Crit Care 1.1 (2013):1-5

<i>Background:</i> Reactive oxygen species play an important role in schsistosomal granuloma formation and disease progression. IL-10 may downregulate cell-mediated immunity. Few data are available about oxidative stress and the immune response of cases of (HCV) coinfected with <i>Schistosoma mansoni</i>. <i>Aims & Methods:</i> To determine pattern of serum IL10, MDA levels as a markers of oxidative stress and total antioxidant in patients with combined HCV and schistosomiasis and their role in disease progression. 60 patients were classified into: Group 1: 20 patients with schistosomal hepatic fibrosis; group 2: 20 patients with HCV; group 3: 20 patients with combined HCV and schistosomiasis. In addition to 20 controls. Serum levels of IL 10, malonaldehyde (MDA), total anti oxidant capacity (TAC) were determined using chemical methods & HCV RNA titer by RT/PCR, liver biopsy were determined. <i>Results:</i> Serum levels of IL-10 were significantly elevated in combined group more than chronic HCV patients (<i>p</i> < 0.05). Serum levels of IL-10 did not correlate with the different laboratory parameters, ishak score or HCV-RNA viral load for all patients (<i>p</i> > 0.05). Serum levels of MDA were significantly elevated while TAC levels were significantly decreased in all groups (<i>p</i> < 0.001). Serum MDA had significant positive correlation with Ishak score fibrosis in both HCV & co infection groups (<i>p</i> = 0.01 and 0.002 respectively). Mixed group had necro-inflammatory scores significantly higher > HCV alone (<i>p</i> = 0.03). Serum TAC showed negative correlation with Ishak score in the mixed group (<i>p</i> = 0.018). In stepwise multiple regression analysis the MDA was the predictor for liver fibrosis in both HCV and co-infected groups. <i>Conclusion:</i> Schistosomiasis triggers a Th2 type immune response and triggers an increase in MDA.