PRODUCTION AND CHARACTERIZATION OF ALKALINE THERMOSTABLE PROTEASE FROM NEWLY ISOLATED BACILLUS SP.

Patil C.S.; Gangawane A.K.; Shankerappa S. Hatti

doi:http://dx.doi.org/10.9735/0976-8823.1.1.9-17

PRODUCTION AND CHARACTERIZATION OF ALKALINE THERMOSTABLE PROTEASE FROM NEWLY ISOLATED BACILLUS SP.

Patil C.S.¹*, Gangawane A.K.², Shankerappa S. Hatti³
¹Department of Biotechnology, B.V. Bhoomareddy College, Bidar-585 403, Karnataka, India
²Department of Biotechnology, School of Biomedical sciences, MGMIHS, Sector -18, Kamothe, Navi Mumbai, 410209, MS, India
³Department of Zoology, Gulbarga University Gulbarga-585106, India
* Corresponding Author : drcspatil1251@yahoo.co.in

Received : - Accepted : - Published : 15-06-2010
Volume : 1 Issue : 1 Pages : 9 - 17
J Plant Genom 1.1 (2010):9-17
DOI : http://dx.doi.org/10.9735/0976-8823.1.1.9-17

Cite - MLA : Patil C.S., et al "PRODUCTION AND CHARACTERIZATION OF ALKALINE THERMOSTABLE PROTEASE FROM NEWLY ISOLATED BACILLUS SP.." Journal of Plant Genomics 1.1 (2010):9-17. http://dx.doi.org/10.9735/0976-8823.1.1.9-17

Cite - APA : Patil C.S., Gangawane A.K., Shankerappa S. Hatti (2010). PRODUCTION AND CHARACTERIZATION OF ALKALINE THERMOSTABLE PROTEASE FROM NEWLY ISOLATED BACILLUS SP.. Journal of Plant Genomics, 1 (1), 9-17. http://dx.doi.org/10.9735/0976-8823.1.1.9-17

Cite - Chicago : Patil C.S., Gangawane A.K., and Shankerappa S. Hatti "PRODUCTION AND CHARACTERIZATION OF ALKALINE THERMOSTABLE PROTEASE FROM NEWLY ISOLATED BACILLUS SP.." Journal of Plant Genomics 1, no. 1 (2010):9-17. http://dx.doi.org/10.9735/0976-8823.1.1.9-17

Copyright : © 2010, Patil C.S., et al, Published by Bioinfo Publications. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

Abstract

An inducible protease is produced by Bacillus sp. in undefined feather medium. Under submerged fermentation condition high level of protease production occurred at 45oC after 36 h at pH 10, with continuous agitation (180 rpm). The presence of carbon source in feather medium suppressed the enzyme production, while 0.1% yeast extract enhanced the production. The purified enzyme showed maximum protease activity at temperature 650C and at pH-10.The enzyme was monomeric and has molecular weight of approximately 66 kDa (SDS-PAGE).The enzyme may belong to serine protease group as it is completely inhibited by PMSF. Presence of metal ions such as Ca2+, Mg2+, Co2+, Ba2+ stimulated, while Hg2+, Pb2+, Zn2+, Fe2+ decreased the activity. The results indicate Bacillus sp. is a highly useful organism for feather meal production and in leather industry.

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