EVALUATING THE CHOLESTEROL LOWERING AND CYTOTOXICITY OF ZARAGOZIC ACID, A NOVEL THERAPEUTIC AGENT ISOLATED FROM Coprophilous fungi

Sharma R.J.¹*, Patwardhan D.G.², Chaphalkar S.R.^3
1VidyaPratishthan’s Arts, Science and Commerce College, Baramati 413133, India.
²VidyaPratishthan’s Arts, Science and Commerce College, Baramati 413133, India.
³VidyaPratishthan’s Arts, Science and Commerce College, Baramati 413133, India.
* Corresponding Author : raajesh_sharma2004@yahoo.com

Received : -     Accepted : -     Published : 15-06-2010
Volume : 2     Issue : 1       Pages : 5 - 8
Int J Pharmaceut Anal 2.1 (2010):5-8
DOI : http://dx.doi.org/10.9735/0975-3079.2.1.5-8

Keywords : Zaragozic acid, Coprophilous fungi , MTT assay.
Conflict of Interest : None declared

Coprophilous fungi were screened for the production of zaragozic acid. Out of the nine isolates obtained three isolates showed acid production during secondary screening followed by its purification using organic solvents. The presence of zaragozic acid was confirmed by antimony trichloride test (SbCl3) and its _max at 190 and 249nm. Further the concentration of zaragozic acid was determined by titrometric analysis, all the six isolates produced 75-80 mg ⁄ L of acid .Cell titer 96™ AQueous one solution reagent from Promege, USA was used to check the cytotoxicity of zaragozic acid. It was found that dosage up to 40 μl of cells did not prove toxic to the cells. The zaragozic acid was then checked for its cholesterol reduction activity. The cholesterol lowering effect of zaragozic acid on cholesterol synthesis was confirmed, 0.8 μg of cholesterol per gm of tissue was reduced by using 30 μl of zaragozic acid.

[1] Bergstorm J.D. et al. (1993) Proc. Natl. Acad. Sci. USA., 80-84  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[2] Bills Gerald, Diez F. et al. (1993) Sporomiella intermedia and process there from-Patent EP0525846  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[3] Tatsuo Tanimoto et al., (1997)) The Journal of Antibiotics 50 (5), 390-394.  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[4] Richard G Bostedor, et al. (1997) The Journal of Biological Chemistry. 272 (14), 9197-9203  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[5] Claude Dufresne, et al. (1993) Cholesterol lowering compounds-5260332.United States Patent  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[6] Eric Voight (2002) Zaragozic acids: Strategy and Discovery in Total Synthesis.3rd year Organic Seminar  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[7] Bergstorm James D., et al. (1995) Annual Review of Microbiology  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[8] Sharma R. J., et al (2010) International Journal of Biotechnology Applications, 2 (1), 01-05.  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[9] De Hoff J.L., Davidson L.M. et al. Clinical Chemistry, 24, 433-435  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus  

[10] Ireland J. T. (1941) Biochem., 35.  
» CrossRef   » Google Scholar   » PubMed   » DOAJ   » CAS   » Scopus