Waghmare S.B.1*, Padwal N.D.2, Jadhav B.V.3
1Helminth Research Lab, Deptt of Zoology, Dr.B.A.M. University, Aurangabad-413004
2Helminth Research Lab, Deptt of Zoology, Dr.B.A.M. University, Aurangabad-413004
3Helminth Research Lab, Deptt of Zoology, Dr.B.A.M. University, Aurangabad-413004
* Corresponding Author : sachinash_red@yahoo.co.in
Received : - Accepted : - Published : 15-06-2009
Volume : 1 Issue : 1 Pages : 1 - 6
Int J Pharmaceut Anal 1.1 (2009):1-6
DOI : http://dx.doi.org/10.9735/0975-3079.1.1.1-6
Conflict of Interest : None declared
Biochemical profiles are subject of variation. Physiological state of the host exhibits profound influence on biochemical individuality of parasite. Methods for various biochemical assays which have been described in many journals are mainly pertaining to mammalian model. The same may not be good for other animal model particularly for helminth parasites. In view of above mentioned facts there is a need to evaluate various existing methods and also to modify the method suitably, to cope up with complexities of biochemical profiles of helminth tissue. The present investigation is planed to evaluate a suitable method related to estimate the total protein quantitatively in the cestode Cotugnia digonopora. Therefore in present investigation is aimed to know the biochemical variability of certain selected biochemical assay related to total protein of cestode Cotugnia digonopora occurred in domestic fowl Gallus gallus domesticus in Maharashtra, India. In the light of present findings it would seem to logical to recommend that a Biuret assay for the cestode parasites, when compared to Folin-Phenol method.
[1] Agosin M. (1957) Exp. Parasitol, 6,586-
593
» CrossRef » Google Scholar » PubMed » DOAJ » CAS » Scopus
[2] Campbell J.W. (1960) J.Parasitol.
46,848
» CrossRef » Google Scholar » PubMed » DOAJ » CAS » Scopus
[3] Helberg P. (1973) Laboratory technique
and rhymatology, in: “Biological
aspects of Circadian Rhythons”, (Ed
.Mills, J.M.), Plenum Press, New
York, 319
» CrossRef » Google Scholar » PubMed » DOAJ » CAS » Scopus
[4] Hawk P.W. (1971) Physiological
chemistry, Tata McGraw Hill
Publishing Co. Ltd
» CrossRef » Google Scholar » PubMed » DOAJ » CAS » Scopus
[5] Kuklin V.V. and Kuklina M.M. (2005)
Gel’minty ptits Barentseva morya:
fauna, ekologiya, vliyanie na
khozyaev (Helminthes of the Birds of
the Barents Sea: Fauna, Ecology,
and Impact on Hosts), Apatity: Izd-vo
KNTs Ross. Akad. Nauk
» CrossRef » Google Scholar » PubMed » DOAJ » CAS » Scopus
[6] Madhava Reddy B, (1981) Studies on
biochemical and physiological
aspects of the two poultry cestodes
cotugnia digonopora and Raillietina
tetragona. Ph.D.thesis, Osmania
University, India.
» CrossRef » Google Scholar » PubMed » DOAJ » CAS » Scopus
[7] Patwari Raghavendra Rao (1982)
Studies on some biochemical and
physiological aspects of two
cestodes. Ph.D.thesis, Osmania
University, India
» CrossRef » Google Scholar » PubMed » DOAJ » CAS » Scopus
[8] Reid W.M. (1942) J.Parasit, 28, 319-340
» CrossRef » Google Scholar » PubMed » DOAJ » CAS » Scopus
[9] Strobel H.H. (1965) Introduction in
“Instrumental methods of
experimental Biology” (Ed. Newman,
D.W.), The Mac Millan Company,
New York, 1-12
» CrossRef » Google Scholar » PubMed » DOAJ » CAS » Scopus
[10] William R.J. (1956) Biochemical
individuality, Willey, New York
» CrossRef » Google Scholar » PubMed » DOAJ » CAS » Scopus