MOLECULAR PHYLOGENETIC STUDY OF LUFFA TUBEROSA ROXB. (CUCURBITACEAE) BASED ON INTERNAL TRANSCRIBED SPACER (ITS) SEQUENCES OF NUCLEAR RIBOSOMAL DNA AND ITS SYSTEMATIC IMPLICATION

Ajmal Ali M.¹*, Karuppusamy S.², Fahad M. Al-Hemaid^3
1Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia
²Department of Botany, The Madura College, Madurai, Tamil Nadu, India.
³Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia
* Corresponding Author : ajmalpdrc@gmail.com

Received : -     Accepted : -     Published : 21-12-2010
Volume : 2     Issue : 2       Pages : 42 - 60
Int J Bioinformatics Res 2.2 (2010):42-60
DOI : http://dx.doi.org/10.9735/0975-3087.2.2.42-60

Keywords : Luffa tuberosa, Momordica, Cucurbitaceae, ITS, nuclear ribosomal DNA
Conflict of Interest : None declared

The phylogenetic position of long been debatable species Luffa tuberosa was inferred in the present study using ITS sequence of nuclear ribosomal DNA data. The study sampled a total number of 16 accessions which include five accessions of Luffa (under four species i.e. Luffa acutangula, L. cylindrica, L. aegyptiaca and L. tuberosa), nine accessions of Momordica (under eight species i.e. M. angustisepala, M. balsamina, M. cabraei, M. charantia, M. charantia subsp. macroloba, M. cissoides, M. cochinchinensis, M. dioica and M. foetida) and two accessions of Trichosanthes under two species (i.e. T. lepiniana and T. tricuspidata). The sequence data analysis clearly reveals nesting of Luffa tuberosa within the clade of Momordica, thus, we herein support the inclusion of Luffa tuberosa into the genus Momordica as M. tuberosa (Roxb.) Cogn.

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