THE EXTRACTION OF FIBROIN PROTEIN FROM BOMBYX MORI SILK COCOON: OPTIMIZATION OF PROCESS PARAMETERS

Mahesh Kumar Sah¹, Arvind Kumar², Pramanik K.³*
¹Department of Biotechnology and Medical Engineering, National Institute of Technology Rourkela-769 008 Orissa, India.
²Department of Chemical Engineering, National Institute of Technology, Rourkela-769 008 Orissa, India.
³Department of Biotechnology and Medical Engineering, National Institute of Technology Rourkela-769 008 Orissa, India.
* Corresponding Author : pearlusbiotech@gmail.com

Received : -     Accepted : -     Published : 21-12-2010
Volume : 2     Issue : 2       Pages : 33 - 41
Int J Bioinformatics Res 2.2 (2010):33-41
DOI : http://dx.doi.org/10.9735/0975-3087.2.2.33-41

Keywords : Bombyx mori; Silk Fibroin; Box-Behnken Design; Response Surface Methodology; Design of Experiments
Conflict of Interest : None declared

Optimization of protein extraction using silk cocoon was carried out by the design of experiment (DOE) to obtain the response surface methodology ( RSM ). Box-Behnken rotatable design was used to create an experimental program to provide data to model the effects of various factors on protein extraction efficiency. The variables chosen were sodium carbonate concentration ( 1 X ), Lithum bromide concentration ( 2 X ) and temperature ( 3 X ). The mathematical relationship between protein extraction efficiency and three significant independent variables can be approximated by a second order quadratic model. RSM was used to describe the individual and interactive effects of three variables at three levels, combined according to a Box-Behnken Design. The coefficient of determination (2 R ) for the model is 0.9761. Probability value ( P < 0.0001) demonstrates a very high significance for the regression model indicating that Box-Behnken Design can be applied to the protein extraction from silk cocoon, and it is an economical way of obtaining the maximum amount of information with the fewest experiments.

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