STUDY OF MOLECULAR SURFACE PROPERTY OF PHOSPHOLIPASE A2 (PLA2) FROM NAJA NAJA

Gomase V.S.¹*, Gangawane A.K.², More A.R.^3
1Department of Bioinformatics, Padmashree Dr. D.Y. Patil University, Navi Mumbai, 400614, India
²Department of Bioinformatics, Padmashree Dr. D.Y. Patil University, Navi Mumbai, 400614, India
³Department of Bioinformatics, Padmashree Dr. D.Y. Patil University, Navi Mumbai, 400614, India
* Corresponding Author : virusgene1@yahoo.co.in

Received : -     Accepted : -     Published : 15-06-2009
Volume : 1     Issue : 1       Pages : 1 - 5
Int J Chem Res 1.1 (2009):1-5
DOI : http://dx.doi.org/10.9735/0975-3699.1.1.1-5

Keywords : phospholipases a2, epitope, hydrophobicity, MHC, surface activity, SVM, PSSM, peptide vaccine
Conflict of Interest : None declared

This study was designed to elucidate the signalling pathways by which secretary phospholipase a2 (sPLA2s) induce in vitro neutrophil migration and PLA2 inhibits platelet aggregation in PRP and explains the decreased clot retraction and retarded and compromised elasticity build up. Naja naja venoms are complex and contain several toxic components, including neurotoxins and phospholipases A2 that cause post-synaptic neuromuscular blockade with respiratory paralysis and cardiac arrest. The antigenic epitopes on phospholipase a2 (Pla2) are important determinant of protection against venom. In this analysis, we found the antigenic epitopes 29-GRGGSGTPVDD-39, 77-TCKGDNNACA-86, of protein called the antigenic determinant or the epitope is sufficient for eliciting the desired immune response. Also predict the MHC binder and these MHC Class peptide segments are from a set of aligned peptides known to bind to a given major histocompatibility complex (MHC) molecule as the predictor of MHC-peptide binding. Binding ability prediction of antigen peptides to MHC class molecules is important in vaccine development. The method integrates prediction of peptide MHC class binding; proteasomal C terminal cleavage efficiency of protein.

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