IN VITRO EVALUATION OF BOTANICALS AND BIO-CONTROL AGENTS AGAINST MUSTARD DAMPING OFF CAUSED BY Pythium aphanidermatum

S. CHANDRAPRAKASH¹*, R.M. SHANGARANARAYANAN², P. RAJESH KUMAR³, REJATH PRAVEEN KUMAR⁴, R. SANTHOSH KUMAR⁵, P. SELVAKUMAR⁶, S. SHANAVAS^7
1Department of Crop Protection, PGP College of Agricultural Sciences, Namakkal, 637405, Tamil Nadu Agricultural University, Coimbatore, 641003, Tamil Nadu, India
²Department of Crop Protection, PGP College of Agricultural Sciences, Namakkal, 637405, Tamil Nadu Agricultural University, Coimbatore, 641003, Tamil Nadu, India
³Department of Crop Protection, PGP College of Agricultural Sciences, Namakkal, 637405, Tamil Nadu Agricultural University, Coimbatore, 641003, Tamil Nadu, India
⁴Department of Crop Protection, PGP College of Agricultural Sciences, Namakkal, 637405, Tamil Nadu Agricultural University, Coimbatore, 641003, Tamil Nadu, India
⁵Department of Crop Protection, PGP College of Agricultural Sciences, Namakkal, 637405, Tamil Nadu Agricultural University, Coimbatore, 641003, Tamil Nadu, India
⁶Department of Crop Protection, PGP College of Agricultural Sciences, Namakkal, 637405, Tamil Nadu Agricultural University, Coimbatore, 641003, Tamil Nadu, India
⁷Department of Crop Protection, PGP College of Agricultural Sciences, Namakkal, 637405, Tamil Nadu Agricultural University, Coimbatore, 641003, Tamil Nadu, India
* Corresponding Author : suriyaprakash235@gmail.com

Received : 09-07-2022     Accepted : 28-07-2022     Published : 30-07-2022
Volume : 14     Issue : 7       Pages : 11544 - 11547
Int J Agr Sci 14.7 (2022):11544-11547

Keywords : Mustard, Damping-off, Pythium aphanidermatum, Bio-agents, Botanicals
Academic Editor : Simona Rainis, S. M. Chavan
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are thankful to Department of Crop Protection, PGP College of Agricultural Sciences, Namakkal, 637405, Tamil Nadu Agricultural University, Coimbatore, 641003, Tamil Nadu, India
Author Contribution : All authors equally contributed

Mustard (Brassica sp.) is one of the important plant species in the family Brassicaceae. Pythium aphanidermatum is a soil-borne plant pathogen that causes damping-off in mustard. Pythium aphanidermatum was isolated from the infected mustard plants, showing typical symptoms of damping off. They were collected from the Kolli hills, Namakkal. Six different solid media were tested for the growth of pathogens, with maximum radial growth of mycelium (topography) on potato dextrose agar (9 cm). Among the two bio-control agents, Trichoderma viride and Bacillus subtilis, Trichoderma viride shows the maximum percent inhibition of mycelial growth (76.33%) and strong antagonistic activity against Pythium aphanidermatum under in vitro conditions. Among the various botanicals (Neem leaf extract, Pungam leaf extract, and Ginger extract) used against Pythium aphanidermatum at different concentrations (5%, 10%, and 15%), Ginger extract with a 15% concentration shows 51.87% inhibition of mycelial growth of Pythium aphanidermatum