ISSN : 0975-3710
EISSN : 0975-9107
A. ANJANI1, J.V. RAMANA2, A.K. SHANKER3*, A.M. LAL4, Y. SATISH5
1Department of Molecular Biology and Biotechnology, SVU Agricultural College, Tirupathi, 517502, Acharya N. G. Ranga Agricultural University, Lam, 522034, India
2Department of Genetics and Plant Breeding, Agricultural College, Bapatla, Acharya N. G. Ranga Agricultural University, Lam, 522034, Guntur, Andhra Pradesh, India
3Principal Scientist, Department of Plant Physiology, ICAR-Central Research Institute for Dryland Agriculture (CRIDA), Hyderabad, 500059, Telangana, India
4Department of Genetics and Plant Breeding, Agricultural College, Bapatla, Acharya N. G. Ranga Agricultural University, Lam, 522034, Guntur, Andhra Pradesh, India
5Senior Scientist, Department of Genetics and Plant Breeding, RARS, Maruteru, Acharya N. G. Ranga Agricultural University, Lam, 522034, Andhra Pradesh, India
* Corresponding Author : arunshank@gmail.com
Received : 01-06-2022 Accepted : 27-06-2022 Published : 30-06-2022
Volume : 14 Issue : 6 Pages : 11391 - 11396
Int J Agr Sci 14.6 (2022):11391-11396
Keywords : AP2 superfamily, Transcription factors, Pearlmillet, In silico mapping, Protein folding
Academic Editor : Anjani Kumari
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are thankful to Department of Molecular Biology and Biotechnology, SVU Agricultural College, Tirupathi, 517502, Acharya N. G. Ranga Agricultural University, Lam, 522034, Guntur, Andhra Pradesh, India
Author Contribution : All authors equally contributed
The APETALA2/ethylene-responsive element binding factor (AP2/EREB) superfamily is one of the largest transcription factor families in plant kingdom which play a predominant role in plant growth processes and are involved in different biotic and abiotic stress responses. A detailed and comprehensive in silico analysis was performed to identify the AP2/ERF transcription factors in pearlmillet which identified 99 AP2/ ERF TFs classified into 19 AP2 family, 62 ERF subfamily, 15 DREB subfamily, 2 RAV family TFs and a soloist. Phylogenetic analysis was performed with the predicted protein sequences and conserved motifs were analysed. Seventy percent AP2 /ERF superfamily genes were found to be localised in nucleus and all of them were mapped onto the seven chromosomes. Physico-chemical parameters were computed and found that thirteen transcription factor proteins were stable. Fold Index was used and found that six transcription factors had no disordered regions and were folded completely. PMERF57 has good stability index and no disordered regions. These identified putative genes could be explored for further analysis
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