EFFECT OF ADDING TAURINE AND TREHALOSE ADDITIVES ON OXIDATIVE STRESS OF SPERMATOZOA OF KANKREJ BULLSEMEN DURING CRYOPRESERVATION

M.R. PATEL¹*, H.K. THUMAR², H.C. NAKHASHI³, B.N. SUTHAR⁴, V.L. SOLANKI⁵, S.M. KALASWA^6
1Department of Veterinary Gynaecology and Obstetrics, Dr V.M. Jhala Clinical Complex, Deesa, Collage of Veterinary Science & A.H., SDAU, Gujarat
²Department of Veterinary Gynaecology and Obstetrics, Dr V.M. Jhala Clinical Complex, Deesa, Collage of Veterinary Science & A.H., SDAU, Gujarat
³Department of Veterinary Gynaecology and Obstetrics, Dr V.M. Jhala Clinical Complex, Deesa, Collage of Veterinary Science & A.H., SDAU, Gujarat
⁴Department of Veterinary Gynaecology and Obstetrics, Dr V.M. Jhala Clinical Complex, Deesa, Collage of Veterinary Science & A.H., SDAU, Gujarat
⁵Department of Veterinary Gynaecology and Obstetrics, Dr V.M. Jhala Clinical Complex, Deesa, Collage of Veterinary Science & A.H., SDAU, Gujarat
⁶Department of Veterinary Gynaecology and Obstetrics, Dr V.M. Jhala Clinical Complex, Deesa, Collage of Veterinary Science & A.H., SDAU, Gujarat
* Corresponding Author : manishpatelvet.22@gmail.com

Received : 06-08-2016     Accepted : 23-07-2017     Published : 12-08-2017
Volume : 9     Issue : 37       Pages : 4569 - 4570
Int J Agr Sci 9.37 (2017):4569-4570

Keywords : Additives, Oxidative stress, Kankrej bull, Cryopreservation
Conflict of Interest : None declared
Acknowledgements/Funding : Author thankful to Department of Veterinary Gynaecology and Obstetrics, Dr V.M. Jhala Clinical Complex, Deesa, Collage of Veterinary Science & A.H., SDAU, Gujarat
Author Contribution : Guidance provided by Dr H.C. Nakhashi and Dr B.N. Suthar and other author help in research work

Total 24 ejaculates, 8 each from 3 mature Kankrej bulls were divided into 3 equal aliquots, the first aliquot was added with TFYG diluent with Taurine (50mM) second with Trehalose (100mM) as semen additives and third serve as a control (without additive) and each aliquot was evaluated at post diluted, post equilibrated and post thawed stages for oxidative parameter viz. Lipid peroxidation (LPO) and Glutathione reductase (GSH). The level of LPO was significantly lower (P <0.05) and GSH were significantly higher (P < 0.05) in presence of Taurine as compared to Trehaloseand control at post thaw stage of semen cryopreservation. It can be concluded that Taurine seems to control the oxidative stress more efficiently as indicated by significant increase in glutathione reductase and decline in malondialdehyde level at post thawed stage of cryopreservation in Kankrej bull semen.

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