SEROPREVALENCE OF BRUCELLOSIS IN SMALL RUMINANTS OF ANAND DISTRICT OF GUJARAT BY VARIOUS SEROLOGICAL METHODS

R.R. PADHER¹*, J.B. NAYAK², ASHISH ROY³, B.B. BHANDERI⁴, B.C. PARMAR⁵, U.P. MISTRY^6
1Department of Veterinary Public Health, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, 388001, Gujarat, India
²Department of Veterinary Public Health, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, 388001, Gujarat, India
³Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, 388001, Gujarat, India
⁴Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, 388001, Gujarat, India
⁵Department of Veterinary Public Health, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, 388001, Gujarat, India
⁶Department of Veterinary Public Health, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, 388001, Gujarat, India
* Corresponding Author : radhapadher@gmail.com

Received : 18-04-2017     Accepted : 05-06-2017     Published : 30-06-2017
Volume : 9     Issue : 30       Pages : 4412 - 4415
Int J Agr Sci 9.30 (2017):4412-4415

Keywords : Brucellosis, Zoonosis, Small ruminants, Seroprevalence
Academic Editor : Das Hemen, Md. Shafiullah Parvej, M Anudeep Reddy, Meena Das, Kurunchi C. Divya, Sujatha Singh, P. Kaur
Conflict of Interest : None declared
Acknowledgements/Funding : The authors are highly thankful to the Dean, College of Veterinary Science and Animal Husbandry, Anand for financial assistance and research facilities to conduct this research work and also thankful to Dr. Snehal Patel, Deputy Director of Animal Husbandry, Annand district for kind support
Author Contribution : all author equally contributed

The present study was taken up to ascertain the seroprevalence of brucellosis in small ruminants of Anand, districts of the Central Gujarat region. A total of 200 serum samples were collected from the goat and sheep of Anand district and subjected to different serological test i.e., Rose Bengal Plate test (RBPT), Standard Tube Agglutination Test (STAT) and Indirect ELISA to detect the brucella antibody. A total 200 sera sample including 100 from goats and 100 from sheep collected from the Anand district. Among 200 sera samples of sheep and goats, 94 (47.00%) samples were found to be positive. 93 sera samples were positive by I-ELISA, 27 by RBPT while 16 by STAT. One RBPT positive goat serum sample was found to be negative by I-ELISA. While species wise incidence was found to be 55 (55.00%) and 38 (38.00%) among goats and sheep, respectively. Fifty six goat sera samples were found to be sero-positive, out of which 55 samples were found to be positive by I-ELISA while 16 by RBPT and 10 by STAT. Among 38 sera samples of sheep found to be positive by I-ELISA while 11 by RBPT and 6 by STAT. Seroprevalence of brucellosis among goat was 16.00%, 10.00% and 55.00% higher than the sheep was 11.00%, 6.00% and 38.00% by RBPT, STAT and I- ELISA respectively. In small ruminantes sex wise seroprevalence was higher in female to be 18%, 13% and 65% then male 9%, 3% and 28% by RBPT, STAT and I-ELISA (Brucella spp.), respectively.

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