STUDIES ON TISSUE CULTURE IN GERBERA (Gerbera jamesonii L.)

T. NAVYA SWETHA¹*, A. GIRWANI², A. MANOHAR RAO³, P. SAIDAIAH^4
1Floriculture and Landscaping Architecture, Sri Konda Laxman Telangana State Horticultural University, Budwel, Hyderabad, 500030, Telangana
²Floriculture and Research Station, Sri Konda Laxman Telangana State Horticultural University, Budwel, Hyderabad, 500030, Telangana
³Department of Horticulture, College of Agriculture, Professor Jayashankar Telangana State Agricultural University, Rajendranagar, Hyderabad, 500030, Telangana
⁴Genetics and Plant Breeding, College of Horticulture, Sri Konda Laxman Telangana State Horticultural University, Budwel, Hyderabad, 500030, Telangana
* Corresponding Author : navyaswetha12@gmail.com

Received : 03-03-2017     Accepted : 19-03-2017     Published : 12-04-2017
Volume : 9     Issue : 17       Pages : 4162 - 4165
Int J Agr Sci 9.17 (2017):4162-4165

Keywords : Gerbera, Tissue culture, Capitulum, Culture establishment
Academic Editor : Lalu Naik Banoth, P. Anitha
Conflict of Interest : None declared
Acknowledgements/Funding : Sincere and gratitude thanks to Sri Konda Laxman Telangana State Horticultural University (SKLTSHU) for providing necessary funding to carry out this study and “AGRI BIOTECH FOUNDATION” Professor Jayashankar Telangana State Agrilcultural University Campus, Rajendranagar, Hyderabad who provided required facility in research work
Author Contribution : Sincere and gratitude thanks to A. Girwani, for her help and extraordinary effort during the achievement of M. Sc. Thesis. A. Manohar Rao, who facilitated the data collection from study areas and P. Saidaiah, who gave technical assistance on culturing of

An attempt has been made to determine the response of capitulum explants of gerbera for micropropagation with 3 mg/L BAP + 0.1 mg/L of IAA was found to be the best medium for culture establishment and primordial emergence (14.66 days). Early shoot initiation was observed on MS medium supplemented with 3mg/L BAP + 0.5mg/L NAA (10.66 days), MS medium supplemented 2 mg/L BAP + 0.5 mg/L NAA was found to produce maximum number of shoots in shortest time (26.00) and 1mg/L BAP + 0.5 mg/L NAA has recorded significantly maximum shoot length (33.00 mm). MS medium supplemented with 2mg/L IBA was best medium for in vitro rooting because it showed highest per cent rooting (83.33%), earlier rooting (19.00 days) with maximum root length (54.66 mm). In vermiculite + vermicompost (1:1, v/v) medium, survival percentage was maximum (80.00%) with maximum plant height (47.00 mm).

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