V. JESWANI1, S.K. SHARMA2, S. RATNAPARKHE3*
1School of Biotechnology, Devi Ahilya Vishwavidyalaya,, Indore, Madhya Pradesh, 452001, India
2Department of Soil Science and Agricultural Chemistry, College of Agriculture, Indore, Madhya Pradesh, 452001, India
3School of Biotechnology, Devi Ahilya Vishwavidyalaya,, Indore, Madhya Pradesh, 452001, India
* Corresponding Author : supriya.ratnaparkhe@gmail.com
Received : 06-10-2016 Accepted : 22-11-2016 Published : 12-12-2016
Volume : 8 Issue : 60 Pages : 3357 - 3360
Int J Agr Sci 8.60 (2016):3357-3360
Keywords : Antibiotic, Antimicrobial activity, Aspergillus niger, Mycoflora, Trichoderma viride, Vermicompost
Conflict of Interest : None declared
Acknowledgements/Funding : This work represents a portion of Vandana Jeswani’s Masters dissertation work and is funded by Department of Biotechnology, Ministry of Science and Technology, New Delhi
Author Contribution : V Jeswani, SK Sharma, and S Ratnaparkhe have equal contribution in design of study. V Jeswani conducted experiments. Supervision and guidance by S Ratnaparkhe and manuscript finalized by V Jeswani, SK Sharma and S Ratnaparkhe
In the present study, three samples of vermicompost obtained from different types of wastes were analyzed for antibiotic producing fungi. The entire mycoflora in broth media of all the three samples and the dominant fungi isolated from them were tested for their antibacterial property against Bacillus subtilis, Escherichia coli, Bacillus licheniformis, Paenibacillus polymyxa and Klebsiella oxytoca. Two of the fungal isolates (Vag002 and Vic003) showed strong antibacterial activity and were selected to undergo further characterization by Morphological examination and Lactophenol cotton blue method. The morphological characteristics of the fungal isolates matched with the description for Trichoderma spp. and Aspergillus spp. Further confirmation by Lactophenol Cotton Blue Test of isolates revealed their identity as Trichoderma viride and Aspergillus niger. The present findings conclude that these microorganisms could be promising source of bioactive compounds and warrant further study.