JACK BEAN UREASE INHIBITION BY CHROMIUM (III)

REZAEI B.G.¹, GONBADIA K.^2
1Chemistry Department, Faculty of Science, Takestan Branch, Islamic Azad University, Takestan, Iran.
²Chemistry Department, Faculty of science, Islamic Azad University, Takestan Branch, Takestan, Iran.

Received : 28-11-2013     Accepted : 18-12-2013     Published : 28-12-2013
Volume : 2     Issue : 1       Pages : 59 - 61
World Res J Pept Protein 2.1 (2013):59-61

Urease activity is often used for characterization of microbial viability in soil. The aim of the investigations was to measure the influence of chromium (III) on urease activity. Urease activity in pure solution was so sensitive for Cr³⁺, which caused inhibition of urease activity significantly. In this paper complexation reaction between Cr³⁺ and Jack bean urease is examined using isothermal titration calorimetry (ITC). It was found that chromium ions acted as a noncooperative inhibitor of JBU, and there is a set of 12 similar and independent binding sites for Cr³⁺ ions. The association equilibrium constant is 6.79×10⁶ L^-1.mol, indicating the strong interaction of Cr³⁺ ion with JBU. The molar enthalpy of binding is DH = 15.10 kJmol^-1.

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