ENDOTHELIAL NITRIC OXIDE SYNTHASE (Enos) GENE POLYMORPHISMS AND THEIR ASSOCIATION WITH TYPE 2 DIABETIC PATIENTS

BHAVSAR K.¹, PATEL A.², LAL S.³, MISTRY K.^4
1Ashok and Rita Patel Inst. of Integrated Study & Res. in Biotechnology and Allied Sciences, New Vallabh Vidyanagar- 388 121, Gujarat, India.
²Ashok and Rita Patel Inst. of Integrated Study & Res. in Biotechnology and Allied Sciences, New Vallabh Vidyanagar- 388 121, Gujarat, India.
³Ashok and Rita Patel Inst. of Integrated Study & Res. in Biotechnology and Allied Sciences, New Vallabh Vidyanagar- 388 121, Gujarat, India.
⁴Ashok and Rita Patel Inst. of Integrated Study & Res. in Biotechnology and Allied Sciences, New Vallabh Vidyanagar- 388 121, Gujarat, India.

Received : 28-11-2013     Accepted : 12-12-2013     Published : 28-12-2013
Volume : 2     Issue : 1       Pages : 17 - 20
World Res J Diabetes 2.1 (2013):17-20

The aim was to find the relationship between eNOS -796T/C in intron 4a/b, Glu298Asp and -786T/C polymorphisms and their association with cardiovascular disease (CVD) in type 2 diabetic patients. Total numbers of 309 patients were taken out of which 105 were controls 63 were cardiovascular disease patient and 53 were type 2 diabetic patients. The analysis of eNOS 4a/b -796T/C, Glu298Asp and -786 T/C polymorphisms was performed by polymerase chain reaction. For Glu298Asp polymorphism there is no individual homozygous for mutant (Asp/Asp genotype) in the study population, the allelic frequency of Asp allele is 0.38% for DM, 0.29% for CVD and p value = 0.022 which is greater than 0.005. The result showed that the allelic frequencies of -796T/C in intron 4 a/b polymorphism is 0.39% for DM, 0.19% for CVD and p value = 0.638 which is not significant. Same with the case of -786 T/C polymorphism, the allelic frequency of CC allele is 0.42% for DM and CVD, which also give p value 0.075 which is also greater than 0.005 thus no significance found among CVD and DM. Thus we conclude that study was conducted in relatively little number of individuals so the association between the eNOS gene and CVD and DM can be well understood by performing case control study using more number of samples.