ISSN : 2249-1813
EISSN : 2249-1821
PATIL V.K.1, GAWAD J.B.2, MHASKE A.J.3, PATIL K.D.4
1Post Graduate Dept. of Pharmaceutical Chemistry, R.C. Patel Inst. of Pharmaceutical Education and Research, Shirpur- 425 405, MS, India.
2Post Graduate Dept. of Pharmaceutical Chemistry, R.C. Patel Inst. of Pharmaceutical Education and Research, Shirpur- 425 405, MS, India.
3Post Graduate Dept. of Pharmaceutical Chemistry, R.C. Patel Inst. of Pharmaceutical Education and Research, Shirpur- 425 405, MS, India.
4Post Graduate Dept. of Pharmaceutics, R.C. Patel Institute of Pharmaceutical Education and Research, Shirpur- 425 405, MS, India.
Received : 05-11-2013 Accepted : 03-12-2013 Published : 24-12-2013
Volume : 2 Issue : 1 Pages : 14 - 18
Bioinfo Pharm Biotechnol 2.1 (2013):14-18
A rapid, precise and specific stability indicating high performance liquid chromatography (RP-HPLC) method was developed for olmesartan medoxomil (OM) and metoprolol succinate (MS). The assay involves the isocratic elution of olmesartan medoxomil and metoprolol succinate in Phenomenex Gemini C18 using mobile phase composition methanol: water (pH 3) in the ratio of 55:45 (v/v). The wavelength of detection is 236 nm. The proposed method can be used for routine quality control in bulk and in dosage forms. The method can also be applied for the stability studies as it separates the constitutions from its degradation products. Retention time of olmesartan medoxomil and metoprolol succinate was found to be 7.9 and 3.4 min, respectively. Linearity was studied in the concentration range of 5 to 25 μg/mL and 15 to 75 μg/mL for olmesartan medoxomil and metoprolol succinate respectively, with a correlation coefficient of 0.999 and 0.997 respectively. The newly developed method was validated according to the ICH guidelines. The stability indicating studies shows the stability of both drugs towards oxidative stress and light; whereas degrades in both acidic and basic conditions.