ELECTROPHORETIC PATTERNS OF MUSCLE PROTEINS ARE USEFUL FOR THE SPECIES IDENTIFICATION

SANTAPE G.B.^1
1Rajarshee Shahu Science College, Chandur (Rly)- 444904, MS, India.

Received : 10-04-2013     Accepted : 30-07-2013     Published : 19-08-2013
Volume : 1     Issue : 1       Pages : 16 - 18
World Res J Cell Biol 1.1 (2013):16-18

Proteins are the macromolecules of life, Their functions are the most diverse of any family of macromolecules. All of those characteristics, nature, and distinctiveness of the proteins give each organism a unique individuality. For the present study L. rohita, C. punctatus and C. batrachus of Amravati region were selected and their muscle protein were analysed by SDS-PAGE, Lamelli (1970) and total protein were estimated by folin phenol method Lowry et al (1951) In fishes, the muscle proteins were estimated from dorsal, gill and caudal regions and it was found that in L. rohita exhibited more proteins in striated muscles removed from all the three regions when compared with those in C. punctatus and C. batrachus. Thus, the present study showed a significant variations in total muscle protein content from selected locomotary organs, which proves that, the organs which are used more store more proteins. Electrophoretic separation of muscle proteins of the selected fishes showed inter specific variations. Distinct differences were observed in the intensity of the stained band. All these observations indicate that, the muscle protein electrophorograms can be used for species identification.